HPA002868

antibody from Atlas Antibodies

Targeting: SDHB SDH, SDH1

Provider product page for HPA002868

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [41]
Comments [0]
Validations
Western blot [1]

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Product number: HPA002868 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA002868, RRID:AB_1079889
Product name: Anti-SDHB
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human SDHB, Gene description: succinate dehydrogenase complex, subunit B, iron sulfur (Ip), Alternative Gene Names: SDH, SDH1, Validated applications: WB, IHC, ICC, Uniprot ID: P21912, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

SDHB protein structure - HPA002868 shown in red.

Submitted references Identification of Succinate Dehydrogenase Gene Variant Carriers by Blood Biomarkers

Gebhardt M, Kunath C, Fröbel D, Funk A, Peitzsch M, Nölting S, Deutschbein T, Januszewicz A, Timmers H, Robledo M, Jahn A, Constantinescu G, Eisenhofer G, Pamporaki C, Richter S
Journal of the Endocrine Society 2024;8(9)

SDHAF2-Linked Metastatic Paraganglioma: A Case Report with Implications for Counseling and Follow-Up of Pathogenic SDHAF2 Variant Carriers

de Jong M, Corssmit E, Jansen J, Potjer T, Bayley J, Hensen E, Rapoport A
Case Reports in Otolaryngology 2024;2024

Fatty acid desaturase 2 determines the lipidomic landscape and steroidogenic function of the adrenal gland

Witt A, Mateska I, Palladini A, Sinha A, Wölk M, Harauma A, Bechmann N, Pamporaki C, Dahl A, Rothe M, Kopaliani I, Adolf C, Riester A, Wielockx B, Bornstein S, Kroiss M, Peitzsch M, Moriguchi T, Fedorova M, Grzybek M, Chavakis T, Mirtschink P, Alexaki V
Science Advances 2023;9(29)

Expanded genetic testing of GIST patients identifies high proportion of non-syndromic patients with germline alterations

Mandelker D, Marra A, Mehta N, Selenica P, Yelskaya Z, Yang C, Somar J, Mehine M, Misyura M, Basturk O, Latham A, Carlo M, Walsh M, Stadler Z, Offit K, Bandlamudi C, Hameed M, Chi P, Reis-Filho J, Ceyhan-Birsoy O
npj Precision Oncology 2023;7(1)

C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization

Desmurs M, Foti M, Raemy E, Vaz F, Martinou J, Bairoch A, Lane L
Molecular and Cellular Biology 2023;35(7):1139-1156

SDHB immunohistochemistry for prognosis of pheochromocytoma and paraganglioma: A retrospective and prospective analysis

Su T, Yang Y, Jiang L, Xie J, Zhong X, Wu L, Jiang Y, Zhang C, Zhou W, Ye L, Ning G, Wang W
Frontiers in Endocrinology 2023;14

Succinate mediates inflammation-induced adrenocortical dysfunction

Mateska I, Witt A, Hagag E, Sinha A, Yilmaz C, Thanou E, Sun N, Kolliniati O, Patschin M, Abdelmegeed H, Henneicke H, Kanczkowski W, Wielockx B, Tsatsanis C, Dahl A, Walch A, Li K, Peitzsch M, Chavakis T, Alexaki V
eLife 2023;12

Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells

Hart M, Quon E, Vigil A, Engstrom I, Newsom O, Davidsen K, Hoellerbauer P, Carlisle S, Sullivan L
eLife 2023;12

Computerized tomography texture analysis of pheochromocytoma: relationship with hormonal and histopathological data

De Leo A, Vara G, Paccapelo A, Balacchi C, Vicennati V, Tucci L, Pagotto U, Selva S, Ricci C, Alberici L, Minni F, Nanni C, Ambrosi F, Santini D, Golfieri R, Di Dalmazi G, Mosconi C
Journal of Endocrinological Investigation 2022;45(10):1935-1944

Androgen-Dependent Prostate Cancer Cells Reprogram Their Metabolic Signature upon GLUT1 Upregulation by Manganese Superoxide Dismutase

Quiros-Gonzalez I, Gonzalez-Menendez P, Mayo J, Hevia D, Artime-Naveda F, Fernandez-Vega S, Fernandez-Fernandez M, Rodriguez-Gonzalez P, Garcia-Alonso J, Sainz R
Antioxidants 2022;11(2):313

Familial wild-type gastrointestinal stromal tumour in association with germline truncating variants in both SDHA and PALB2

Whitworth J, Casey R, Smith P, Giger O, Martin J, Clark G, Cook J, Fernando M, Taniere P, Maher E
European Journal of Human Genetics 2021;29(7):1139-1145

Axl‐inhibitor bemcentinib alleviates mitochondrial dysfunction in the unilateral ureter obstruction murine model

Hoel A, Osman T, Hoel F, Elsaid H, Chen T, Landolt L, Babickova J, Tronstad K, Lorens J, Gausdal G, Marti H, Furriol J
Journal of Cellular and Molecular Medicine 2021;25(15):7407-7417

Metabolomics, machine learning and immunohistochemistry to predict succinate dehydrogenase mutational status in phaeochromocytomas and paragangliomas

Wallace P, Conrad C, Brückmann S, Pang Y, Caleiras E, Murakami M, Korpershoek E, Zhuang Z, Rapizzi E, Kroiss M, Gudziol V, Timmers H, Mannelli M, Pietzsch J, Beuschlein F, Pacak K, Robledo M, Klink B, Peitzsch M, Gill A, Tischler A, de Krijger R, Papathomas T, Aust D, Eisenhofer G, Richter S
The Journal of Pathology 2020;251(4):378-387

The loss of succinate dehydrogenase B expression is frequently identified in hemangioblastoma of the central nervous system

Roh T, Yim H, Roh J, Lee K, Park S, Jeong S, Kim S, Kim J
Scientific Reports 2019;9(1)

Mutations in NDUFS1 Cause Metabolic Reprogramming and Disruption of the Electron Transfer

Ni Y, Hagras M, Konstantopoulou V, Mayr J, Stuchebrukhov A, Meierhofer D
Cells 2019;8(10):1149

MITF controls the TCA cycle to modulate the melanoma hypoxia response

Louphrasitthiphol P, Ledaki I, Chauhan J, Falletta P, Siddaway R, Buffa F, Mole D, Soga T, Goding C
Pigment Cell & Melanoma Research 2019;32(6):792-808

SUN-344 SDHB Immunostaining: Still an Indispensable Tool in Characterising Pheochromocytoma and Paraganglioma

Velusamy A, Moonim M, Hubbard J, McGowan B, Powrie J, Izatt L, Obholzer R, Jacques A, Breen L, Carroll P
Journal of the Endocrine Society 2019;3(Supplement_1)

Genetic profile of Indian pheochromocytoma and paraganglioma patients – A single institutional study

Agarwal G, Rajan S, Valiveru R, Tulsyan S, Agrawal V, Mittal B, Zaidi G, Mayilvaganan S, Mishra A, Agarwal A, Mishra S, Bhatia E
Indian Journal of Endocrinology and Metabolism 2019;23(4):486

Metabolome-guided genomics to identify pathogenic variants in isocitrate dehydrogenase, fumarate hydratase, and succinate dehydrogenase genes in pheochromocytoma and paraganglioma

Richter S, Gieldon L, Pang Y, Peitzsch M, Huynh T, Leton R, Viana B, Ercolino T, Mangelis A, Rapizzi E, Menschikowski M, Aust D, Kroiss M, Beuschlein F, Gudziol V, Timmers H, Lenders J, Mannelli M, Cascon A, Pacak K, Robledo M, Eisenhofer G, Klink B
Genetics in Medicine 2019;21(3):705-717

IDH1 or -2 mutations do not predict outcome and do not cause loss of 5-hydroxymethylcytosine or altered histone modifications in central chondrosarcomas.

Cleven AHG, Suijker J, Agrogiannis G, Briaire-de Bruijn IH, Frizzell N, Hoekstra AS, Wijers-Koster PM, Cleton-Jansen AM, Bovée JVMG
Clinical sarcoma research 2017;7:8

Implications of SDHB genetic testing in patients with sporadic pheochromocytoma

Maignan A, Guerin C, Julliard V, Paladino N, Kim E, Roche P, Castinetti F, Essamet W, Mancini J, Imperiale A, Clifton-Bligh R, Romanet P, Barlier A, Pacak K, Sebag F, Taïeb D
Langenbeck's Archives of Surgery 2017;402(5):787-798

Validation of pathological grading systems for predicting metastatic potential in pheochromocytoma and paraganglioma

Vicha A, Koh J, Ahn S, Kim H, Kim B, Sung T, Kim Y, Hong S, Song D, Lee S
PLOS ONE 2017;12(11):e0187398

Hypoxia potentiates the cytotoxic effect of piperlongumine in pheochromocytoma models

Bullova P, Cougnoux A, Abunimer L, Kopacek J, Pastorekova S, Pacak K
Oncotarget 2016;7(26):40531-40545

NDUFAF5 Hydroxylates NDUFS7 at an Early Stage in the Assembly of Human Complex I

Rhein V, Carroll J, Ding S, Fearnley I, Walker J
Journal of Biological Chemistry 2016;291(28):14851-14860

Deciphering the molecular basis of invasiveness in Sdhb-deficient cells

Loriot C, Domingues M, Berger A, Menara M, Ruel M, Morin A, Castro-Vega L, Letouzé É, Martinelli C, Bemelmans A, Larue L, Gimenez-Roqueplo A, Favier J
Oncotarget 2015;6(32):32955-32965

Urinary Bladder Paragangliomas

Giubellino A, Lara K, Martucci V, Huynh T, Agarwal P, Pacak K, Merino M
American Journal of Surgical Pathology 2015;39(11):1488-1492

SDHB/SDHA immunohistochemistry in pheochromocytomas and paragangliomas: a multicenter interobserver variation analysis using virtual microscopy: a Multinational Study of the European Network for the Study of Adrenal Tumors (ENS@T)

Papathomas T, Oudijk L, Persu A, Gill A, van Nederveen F, Tischler A, Tissier F, Volante M, Matias-Guiu X, Smid M, Favier J, Rapizzi E, Libe R, Currás-Freixes M, Aydin S, Huynh T, Lichtenauer U, van Berkel A, Canu L, Domingues R, Clifton-Bligh R, Bialas M, Vikkula M, Baretton G, Papotti M, Nesi G, Badoual C, Pacak K, Eisenhofer G, Timmers H, Beuschlein F, Bertherat J, Mannelli M, Robledo M, Gimenez-Roqueplo A, Dinjens W, Korpershoek E, de Krijger R
Modern Pathology 2015;28(6):807-821

Tyrosine kinase receptors as molecular targets in pheochromocytomas and paragangliomas

Cassol C, Winer D, Liu W, Guo M, Ezzat S, Asa S
Modern Pathology 2014;27(8):1050-1062

Aberrant DNA hypermethylation of SDHC: a novel mechanism of tumor development in Carney triad

Haller F, Moskalev E, Faucz F, Barthelmeß S, Wiemann S, Bieg M, Assie G, Bertherat J, Schaefer I, Otto C, Rattenberry E, Maher E, Ströbel P, Werner M, Carney J, Hartmann A, Stratakis C, Agaimy A
Endocrine-Related Cancer 2014;21(4):567-577

Paraganglioma and pheochromocytoma upon maternal transmission of SDHDmutations

Bayley J, Oldenburg R, Nuk J, Hoekstra A, van der Meer C, Korpershoek E, McGillivray B, Corssmit E, Dinjens W, de Krijger R, Devilee P, Jansen J, Hes F
BMC Medical Genetics 2014;15(1)

Integrated genomic study of quadruple-WT GIST (KIT/PDGFRA/SDH/RAS pathway wild-type GIST)

Nannini M, Astolfi A, Urbini M, Indio V, Santini D, Heinrich M, Corless C, Ceccarelli C, Saponara M, Mandrioli A, Lolli C, Ercolani G, Brandi G, Biasco G, Pantaleo M
BMC Cancer 2014;14(1)

A MEN1 syndrome with a paraganglioma

Jamilloux Y, Favier J, Pertuit M, Delage-Corre M, Lopez S, Teissier M, Mathonnet M, Galinat S, Barlier A, Archambeaud F
European Journal of Human Genetics 2013;22(2):283-285

Analysis of all subunits, SDHA, SDHB, SDHC, SDHD, of the succinate dehydrogenase complex in KIT/PDGFRA wild-type GIST

Pantaleo M, Astolfi A, Urbini M, Nannini M, Paterini P, Indio V, Saponara M, Formica S, Ceccarelli C, Casadio R, Rossi G, Bertolini F, Santini D, Pirini M, Fiorentino M, Basso U, Biasco G
European Journal of Human Genetics 2013;22(1):32-39

Succinate Dehydrogenase (SDH) D Subunit (SDHD) Inactivation in a Growth-Hormone-Producing Pituitary Tumor: A New Association for SDH?

Stratakis C, Tolis G, Lytras A, Pasini B, Ferrando B, Patronas N, Quezado M, Lyssikatos C, Horvath A, Azevedo M, Ball E, Matro J, de la Luz Sierra M, Nesterova M, Rustin P, Wassif C, Almeida M, Pacak K, Xekouki P
The Journal of Clinical Endocrinology & Metabolism 2012;97(3):E357-E366

Mitochondrial function and content in pheochromocytoma/paraganglioma of succinate dehydrogenase mutation carriers

Rapizzi E, Ercolino T, Canu L, Giaché V, Francalanci M, Pratesi C, Valeri A, Mannelli M
Endocrine-Related Cancer 2012;19(3):261-269

Sumo1-ylation of human spermatozoa and its relationship with semen quality

Marchiani S, Tamburrino L, Giuliano L, Nosi D, Sarli V, Gandini L, Piomboni P, Belmonte G, Forti G, Baldi E, Muratori M
International Journal of Andrology 2011;34(6pt1):581-593

SDHB immunohistochemistry: a useful tool in the diagnosis of Carney–Stratakis and Carney triad gastrointestinal stromal tumors

Gaal J, Stratakis C, Carney J, Ball E, Korpershoek E, Lodish M, Levy I, Xekouki P, van Nederveen F, den Bakker M, O'Sullivan M, Dinjens W, de Krijger R
Modern Pathology 2011;24(1):147-151

A non-catecholamine-producing sympathetic paraganglioma of the spermatic cord: the importance of performing candidate gene mutation analysis

Alataki D, Triantafyllidis A, Gaal J, Rodiou C, Vouros J, Papathanasiou A, Papanicolaou A, Rombis V, de Krijger R
Virchows Archiv 2010;457(5):619-622

SDHA is a tumor suppressor gene causing paraganglioma

Burnichon N, Brière J, Libé R, Vescovo L, Rivière J, Tissier F, Jouanno E, Jeunemaitre X, Bénit P, Tzagoloff A, Rustin P, Bertherat J, Favier J, Gimenez-Roqueplo A
Human Molecular Genetics 2010;19(15):3011-3020

The Warburg Effect Is Genetically Determined in Inherited Pheochromocytomas

Najbauer J, Favier J, Brière J, Burnichon N, Rivière J, Vescovo L, Benit P, Giscos-Douriez I, De Reyniès A, Bertherat J, Badoual C, Tissier F, Amar L, Libé R, Plouin P, Jeunemaitre X, Rustin P, Gimenez-Roqueplo A
PLoS ONE 2009;4(9):e7094

An immunohistochemical procedure to detect patients with paraganglioma and phaeochromocytoma with germline SDHB, SDHC, or SDHD gene mutations: a retrospective and prospective analysis

van Nederveen F, Gaal J, Favier J, Korpershoek E, Oldenburg R, de Bruyn E, Sleddens H, Derkx P, Rivière J, Dannenberg H, Petri B, Komminoth P, Pacak K, Hop W, Pollard P, Mannelli M, Bayley J, Perren A, Niemann S, Verhofstad A, de Bruïne A, Maher E, Tissier F, Méatchi T, Badoual C, Bertherat J, Amar L, Alataki D, Van Marck E, Ferrau F, François J, de Herder W, Peeters M, van Linge A, Lenders J, Gimenez-Roqueplo A, de Krijger R, Dinjens W
The Lancet Oncology 2009;10(8):764-771

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SDHB antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Sample type: Human
Protocol: Protocol