Ab097410

antibody from Aladdin Scientific

Targeting: COX4I1 COX4, COX4-1

Immunoprecipitation

Antibody data

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Validation data

Product number: Ab097410 - Provider product page
Provider: Aladdin Scientific
Product name: Recombinant COX IV Antibody
Antibody type: Recombinant
Description: Rabbit anti Human COX IV Antibody, Recombinant antibody (RR684), could be used for WB, IHC, Flow, IF, ICC, IP and so on.Application:WB: 1/5000 - 1/10000IHC: 1/200 - 1/400Flow: 1/200 - 1/1000IF/ICC: 1/10 - 1/500IP: 1/50
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine
Host: Rabbit
Conjugate: Unconjugated
Antibody clone number: RR684
Vial size: 100_l,10_l,1ml,50_l
Concentration: 0,5 mg/ml
Storage: Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

COX4I1 protein structure - Ab097410 shown in red.

Supportive validation

Submitted by: Aladdin Scientific (provider)
Main image
Experimental details: Recombinant COX IV Antibody (Ab097410) - IF IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Supportive validation

Submitted by: Aladdin Scientific (provider)
Main image
Experimental details: Recombinant COX IV Antibody (Ab097410) - IF IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Supportive validation

Submitted by: Aladdin Scientific (provider)
Main image
Experimental details: Recombinant COX IV Antibody (Ab097410) - IF IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.