PA5-29992

antibody from Invitrogen Antibodies

Targeting: COX4I1 COX4, COX4-1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29992

Provider

Invitrogen Antibodies

Product name

COX4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse muscle, rat muscle, mouse striatal progenitor cells. Predicted reactivity: Rabbit (80%), Rhesus Monkey (93%), Chimpanzee (100%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.42 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer's Disease Models.

Zhong M, Kou H, Zhao P, Zheng W, Xu H, Zhang X, Lan W, Guo C, Wang T, Guo F, Wang Z, Gao H
Frontiers in aging neuroscience 2021;13:660249

---

FTY720 Prevents Spatial Memory Impairment in a Rat Model of Chronic Cerebral Hypoperfusion via a SIRT3-Independent Pathway.

Zhang M, Hu Y, Zhang J, Zhang J
Frontiers in aging neuroscience 2020;12:593364

---

Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes.

Beutner G, Eliseev RA, Porter GA Jr
PloS one 2014;9(11):e113330

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COX4 Polyclonal Antibody detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COX4 stained by COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Representative SIRT3 (A) , acetylated SOD2 K68 (Ac-SOD2 K68) (B) expressions, SOD2 activity (C) in the hippocampus of rats in each group. SIRT3 and SOD2 K68 expression in the hippocampus were normalized to loading of COX IV and SOD2, respectively, and were expressed as fold of sham-NS. Data were shown as mean +- S.E.M. n = 4-5 rats for each group. * p < 0.05 compared with the sham-NS group.