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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29992 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse muscle, rat muscle, mouse striatal progenitor cells.
- Concentration
- 0.42 mg/mL
Submitted references Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer's Disease Models.
FTY720 Prevents Spatial Memory Impairment in a Rat Model of Chronic Cerebral Hypoperfusion via a SIRT3-Independent Pathway.
Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes.
Zhong M, Kou H, Zhao P, Zheng W, Xu H, Zhang X, Lan W, Guo C, Wang T, Guo F, Wang Z, Gao H
Frontiers in aging neuroscience 2021;13:660249
Frontiers in aging neuroscience 2021;13:660249
FTY720 Prevents Spatial Memory Impairment in a Rat Model of Chronic Cerebral Hypoperfusion via a SIRT3-Independent Pathway.
Zhang M, Hu Y, Zhang J, Zhang J
Frontiers in aging neuroscience 2020;12:593364
Frontiers in aging neuroscience 2020;12:593364
Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes.
Beutner G, Eliseev RA, Porter GA Jr
PloS one 2014;9(11):e113330
PloS one 2014;9(11):e113330
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX4 using Various whole cell extracts (30 µg). Samples were loaded onto a 15% SDS-PAGE gel and probed with a COX4 polyclonal antibody (Product # PA5-29992) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4) and LNCaP (Lane 5). The blot was probed with Anti-COX4 Polyclonal Antibody (Product # PA5-29992, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 17 kDa band corresponding to COX4 was detected across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX4 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a COX4 Polyclonal Antibody (Product # PA5-29992) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COX4 Polyclonal Antibody (Product # PA5-29992). Sample (50 µg of whole cell lysate). Lane A: mouse muscle. 12% SDS PAGE. COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COX4 Polyclonal Antibody (Product # PA5-29992). Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COX4 Polyclonal Antibody (Product # PA5-29992). Sample (50 µg of whole cell lysate). Lane A: rat muscle. 12% SDS PAGE. COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COX4 Polyclonal Antibody detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COX4 stained by COX4 Polyclonal Antibody (Product # PA5-29992) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Representative SIRT3 (A) , acetylated SOD2 K68 (Ac-SOD2 K68) (B) expressions, SOD2 activity (C) in the hippocampus of rats in each group. SIRT3 and SOD2 K68 expression in the hippocampus were normalized to loading of COX IV and SOD2, respectively, and were expressed as fold of sham-NS. Data were shown as mean +- S.E.M. n = 4-5 rats for each group. * p < 0.05 compared with the sham-NS group.
