Antibody data
- Product number
- PA5-29992
- Provider
- Invitrogen Antibodies
- Product name
- Anti-COX4 Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-29992
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-29992 targets COX4 in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples. The PA5-29992 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 169 of Human COX4. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.42 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX4 using Various whole cell extracts (30 µg). Samples were loaded onto a 15% SDS-PAGE gel and probed with a COX4 polyclonal antibody (Product # PA5-29992) at a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX4 using 50 µg of mouse muscle lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a COX4 polyclonal antibody (Product # PA5-29992) at a dilution of 1:500.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX4 using 50 µg of rat muscle lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a COX4 polyclonal antibody (Product # PA5-29992) at a dilution of 1:500.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4) and LNCaP (Lane 5). The blot was probed with Anti-COX4 Polyclonal Antibody (Product # PA5-29992, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 17 kDa band corresponding to COX4 was detected across the cell lines tested.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COX4 in paraffin-embedded HS578T xenograft using a COX4 polyclonal antibody (Product # PA5-29992) at a dilution of 1:250.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COX4 in methanol-fixed HeLa cells using a COX4 polyclonal antibody (Product # PA5-29992) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive data in Antibodypedia
Data presented on provider website
