TA500960

antibody from OriGene

Targeting: BHMT BHMT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500960

Provider

OriGene

Proper citation

OriGene Cat#TA500960, RRID:AB_11125640

Product name

Anti-BHMT mouse monoclonal antibody, clone OTI10B3 (formerly 10B3)

Antibody type

Monoclonal

Description

Anti-BHMT mouse monoclonal antibody, clone OTI10B3 (formerly 10B3)

Host

Mouse

Conjugate

Unconjugated

Epitope

BHMT

Isotype

IgG

Antibody clone number

OTI10B3

Vial size

100 µl

Concentration

0.94 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT .

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT .

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BHMT mouse monoclonal antibody (TA500960) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT (RC203148).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-BHMT antibody(TA500960),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC