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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21470 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BHMT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, mouse liver. Predicted reactivity: Mouse (95%), Rat (93%), Zebrafish (82%), Xenopus laevis (87%), Pig (95%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BHMT was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BHMT Polyclonal Antibody (Product # PA5-21470) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BHMT Polyclonal Antibody (Product # PA5-21470). Sample (30 µg whole cell lysate). A: Hep G2. 10% SDS PAGE. BHMT Polyclonal Antibody (Product # PA5-21470) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of betaine-homocysteine methyltransferase in methanol-fixed A431 cells using a betaine-homocysteine methyltransferase polyclonal antibody (Product # PA5-21470) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using BHMT antibody (Product # PA5-21470) at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BHMT Polyclonal Antibody detects BHMT protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. BHMT Polyclonal Antibody (Product # PA5-21470) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
