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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX114873 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114873, RRID:AB_11164243
- Product name
- UQCRC2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis.
Optic atrophy 1 mediates coenzyme Q-responsive regulation of respiratory complex IV activity in brain mitochondria.
The short variant of the mitochondrial dynamin OPA1 maintains mitochondrial energetics and cristae structure.
Imami K, Milek M, Bogdanow B, Yasuda T, Kastelic N, Zauber H, Ishihama Y, Landthaler M, Selbach M
Molecular cell 2018 Oct 4;72(1):84-98.e9
Molecular cell 2018 Oct 4;72(1):84-98.e9
Optic atrophy 1 mediates coenzyme Q-responsive regulation of respiratory complex IV activity in brain mitochondria.
Takahashi K, Ohsawa I, Shirasawa T, Takahashi M
Experimental gerontology 2017 Nov;98:217-223
Experimental gerontology 2017 Nov;98:217-223
The short variant of the mitochondrial dynamin OPA1 maintains mitochondrial energetics and cristae structure.
Lee H, Smith SB, Yoon Y
The Journal of biological chemistry 2017 Apr 28;292(17):7115-7130
The Journal of biological chemistry 2017 Apr 28;292(17):7115-7130
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE GTX114873 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membranes were blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000 and competitor's antibody (14742-1-AP) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using UQCRC2(GTX114873) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC2 antibody immunoprecipitates UQCRC2 protein in IP experiments.IP samples: HepG2 whole cell extractA. 35 £gg HepG2 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of UQCRC2 protein by 4 £gg UQCRC2 antibody (GTX114873)10 % SDS-PAGEThe immunoprecipitated UQCRC2 protein was detected by UQCRC2 antibody (GTX114873) diluted at 1:5000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UQCRC2 antibody detects UQCRC2 protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. UQCRC2 antibody (GTX114873) dilution: 1:250.
