PA5-52500

antibody from Invitrogen Antibodies

Targeting: UQCRC2 QCR2, UQCR2

Antibody data

Submit

Validation data

Product number: PA5-52500 - Provider product page
Provider: Invitrogen Antibodies
Product name: UQCRC2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: IKAGSRYEDF SNLGTTHLLR LTSSLTTKGA SSFKITRGIE AVGGKLSVTA TRENMAYTVE CLRGDVDILM EFLLNVTTAP EFRRWEVADL QPQLKIDKAV AFQNPQTHVI ENLHAAAYRN ALANPLYCPD YRIGKVTSEE LHYFVQN
Concentration: 0.1 mg/mL

UQCRC2 protein structure - PA5-52500 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of UQCRC2 was achieved by transfecting Hep G2 with UQCRC2 specific siRNAs (Silencer® select Product # s14703, s14702). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the UQCRC2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with UQCRC2 Polyclonal Antibody (Product # PA5-52500, 0.4ug/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to UQCRC2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of UQCRC2 using UQCRC2 Polyclonal Antibody (Product # PA5-52500) (A) shows similar pattern to an independent UQCRC2 Polyclonal Antibody (B).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using UQCRC2 Polyclonal Antibody (Product # PA5-52500) and a 48 kDa band corresponding to UQCRC2 was observed in HepG2, HEK-293, A-431, U-2 OS and Mouse Heart and Mouse Kidney. Certain uncharacterized bands at ~70-80 kDa were observed in certain samples. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), A-431 (Lane 3), U-2 OS (Lane 4), Mouse Heart (Lane 5) and Mouse Kidney (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of UQCRC2 in human cerebral cortex using UQCRC2 Polyclonal Antibody (Product # PA5-52500).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of UQCRC2 in human heart muscle and pancreas tissues using UQCRC2 Polyclonal Antibody (Product # PA5-52500). Corresponding UQCRC2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of UQCRC2 in human cerebral cortex, heart muscle, liver and pancreas using UQCRC2 Polyclonal Antibody (Product # PA5-52500) (A) shows similar protein distribution across tissues to an independent UQCRC2 Polyclonal Antibody (B).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of UQCRC2 in human pancreas using UQCRC2 Polyclonal Antibody (Product # PA5-52500) shows low expression as expected.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of UQCRC2 in human heart muscle using UQCRC2 Polyclonal Antibody (Product # PA5-52500) shows high expression.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of UQCRC2 in human liver using UQCRC2 Polyclonal Antibody (Product # PA5-52500).