Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-52500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UQCRC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IKAGSRYEDF SNLGTTHLLR LTSSLTTKGA SSFKITRGIE AVGGKLSVTA TRENMAYTVE CLRGDVDILM EFLLNVTTAP EFRRWEVADL QPQLKIDKAV AFQNPQTHVI ENLHAAAYRN ALANPLYCPD YRIGKVTSEE LHYFVQN Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 85%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of UQCRC2 was achieved by transfecting Hep G2 with UQCRC2 specific siRNAs (Silencer® select Product # s14703, s14702). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the UQCRC2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with UQCRC2 Polyclonal Antibody (Product # PA5-52500, 0.4ug/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to UQCRC2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UQCRC2 using UQCRC2 Polyclonal Antibody (Product # PA5-52500) (A) shows similar pattern to an independent UQCRC2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using UQCRC2 Polyclonal Antibody (Product # PA5-52500) and a 48 kDa band corresponding to UQCRC2 was observed in HepG2, HEK-293, A-431, U-2 OS and Mouse Heart and Mouse Kidney. Certain uncharacterized bands at ~70-80 kDa were observed in certain samples. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), A-431 (Lane 3), U-2 OS (Lane 4), Mouse Heart (Lane 5) and Mouse Kidney (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of UQCRC2 in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using an UQCRC2 Polyclonal Antibody (Product # PA5-52500).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UQCRC2 in human cerebral cortex using UQCRC2 Polyclonal Antibody (Product # PA5-52500).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UQCRC2 in human heart muscle and pancreas tissues using UQCRC2 Polyclonal Antibody (Product # PA5-52500). Corresponding UQCRC2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UQCRC2 in human cerebral cortex, heart muscle, liver and pancreas using UQCRC2 Polyclonal Antibody (Product # PA5-52500) (A) shows similar protein distribution across tissues to an independent UQCRC2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UQCRC2 in human pancreas using UQCRC2 Polyclonal Antibody (Product # PA5-52500) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UQCRC2 in human heart muscle using UQCRC2 Polyclonal Antibody (Product # PA5-52500) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UQCRC2 in human liver using UQCRC2 Polyclonal Antibody (Product # PA5-52500).