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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- MA5-37840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- E2F6 Recombinant Rabbit Monoclonal Antibody (9I5M0)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: 293T, HepG2, RD Immunogen sequence: LDGIDLVEKK SKNHIRWIGS DLSNFGAVPQ QKKLQEELSD LSAMEDALDE LIKDCAQQLF ELTDDKENER LAYVTYQDIH SIQAFHEQIV IAVKAPAETR LDVPAPREDS ITVHIRSTNG PIDVYLCEVE QGQTSNKRSE GVGTSSSEST HPEGPEEEEN PQQSEELLEV SN
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9I5M0
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of E2F6 in extracts of various cell lines. Samples were incubated with E2F6 Monoclonal antibody (Product # MA5-37840) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of E2F6 in 300 μg extracts of 293T cells. Samples were precipitated with 3 μg E2F6 Monoclonal antibody (Product # MA5-37840). Western blot was performed from the immunoprecipitate using E2F6 Monoclonal antibody (Product # MA5-37840) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of E2F6 in extracts of 293T cells. Samples were incubated with E2F6 Monoclonal antibody (Product # MA5-37840) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
