Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PA1123 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Matrix metalloproteinase-16 MMP16 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for MT3 MMP/MMP16 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MT3 MMP/MMP16 information: Molecular Weight: 69521 MW; Subcellular Localization: Isoform Long: Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Localized at the cell surface of melanoma cells; Tissue Specificity: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references miRNA 146b-5p protects against atherosclerosis by inhibiting vascular smooth muscle cell proliferation and migration.
MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma.
Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters.
Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16.
MMP-14 can serve as a prognostic marker in patients with supraglottic cancer.
microRNA-146b inhibits glioma cell migration and invasion by targeting MMPs.
Sun D, Xiang G, Wang J, Li Y, Mei S, Ding H, Yan J
Epigenomics 2020 Dec;12(24):2189-2204
Epigenomics 2020 Dec;12(24):2189-2204
MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma.
Xue Z, Wu X, Chen X, Luo Q
Cancer medicine 2016 Sep;5(9):2459-68
Cancer medicine 2016 Sep;5(9):2459-68
Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters.
Xiao T, Zeng O, Luo J, Wu Z, Li F, Yang J
Bio-medical materials and engineering 2015;26 Suppl 1:S2033-9
Bio-medical materials and engineering 2015;26 Suppl 1:S2033-9
Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16.
Lin F, Wang X, Jie Z, Hong X, Li X, Wang M, Yu Y
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2011 Aug;31(4):509
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2011 Aug;31(4):509
MMP-14 can serve as a prognostic marker in patients with supraglottic cancer.
Zhang H, Liu M, Sun Y, Lu J
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 2009 Sep;266(9):1427-34
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 2009 Sep;266(9):1427-34
microRNA-146b inhibits glioma cell migration and invasion by targeting MMPs.
Xia H, Qi Y, Ng SS, Chen X, Li D, Chen S, Ge R, Jiang S, Li G, Chen Y, He ML, Kung HF, Lai L, Lin MC
Brain research 2009 May 7;1269:158-65
Brain research 2009 May 7;1269:158-65
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of MMP16 using anti- MMP16 antibody (PA1123). MMP16 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image