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Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-76959 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GLP2R Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references [Gly²]-GLP-2, But Not Glucagon or [D-Ala²]-GLP-1, Controls Collagen Crosslinking in Murine Osteoblast Cultures.
Mieczkowska A, Bouvard B, Legrand E, Mabilleau G
Frontiers in endocrinology 2021;12:721506
Frontiers in endocrinology 2021;12:721506
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 1 Expression and activation of proglucagon-derived peptide receptors in murine osteoblasts. (A) Relative expression of glucagon receptor ( Gcgr ), glucagon-like peptide-1 receptor ( Glp1r ), and glucagon-like peptide-2 receptor ( Glp2r ) was investigated by qPCR. CHO-K1 cells transfected with the empty pcDNA3.1 vector was used as negative control. CHO-K1 cells transfected with the pcDNA 3.1 vector encoding the Gcgr , Glp1r , or Glp2r sequence were used as positive control. Expression in MC3T3-E1 cells was used to assess expression in osteoblast cells. (B) Presence of GCGr, GLP-1r, and GLP-2r was determined by Western blot with commercially available antibodies. Arrowheads point out at the theoretical location of receptor. (C) Intracellular levels of cAMP were determined in MC3T3-E1 cells 45 min after treatment with 50 pM of GCG, [D-Ala2]-GLP-1, or [Gly2]-GLP-2. (D) Phospho-proteome array in MC3T3-E1 cells treated with either saline or 50 pM of GLP-2 or its long-lasting analog [Gly2]-GLP-2. (E) Intracellular levels of cAMP were determined in MC3T3-E1 cells 45 min after treatment with 50 pM of GLP-2 or [Gly2]-GLP-2. * p < 0.05 and ** p < 0.01 vs. saline-treated cultures.
