AP6287a

antibody from Abgent

Targeting: ATF4 CREB-2, TAXREB67, TXREB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6287a

Provider

Abgent

Proper citation

Abgent Cat#AP6287a, RRID:AB_2058602

Product name

ATF4 Antibody (S245)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245) (Cat. #AP6287a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of ATF4 Antibody (S245) Pab (Cat. #AP6287a) pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000