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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [2]
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- Product number
- PA5-27576 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATF4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HeLa (4 µg/mL tunicamycin treatment for 16 hr). Predicted reactivity: Mouse (83%), Rat (85%), Pig (89%), Sheep (86%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATF4 in HeLa cells mock (left) and HeLa cells treated with 2 µg/mL Tunicamycin for 8hrs (right). Cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using ATF4 Polyclonal Antibody (Product # PA5-27576) (Green) at a dilution of 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATF4 in HeLa cells mock (left) and HeLa cells treated with 2 µg/mL Tunicamycin for 8hrs (right). Cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using ATF4 Polyclonal Antibody (Product # PA5-27576) (Green) at a dilution of 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous ATF4 protein at specific gene loci using Anti-ATF4 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-ATF4 Rabbit Polyclonal Antibody (Product # PA5-27576, 3 µg) on sheared chromatin from 2 million MDA-MB-231 cells treated with Cobalt chloride (150 µM for 24 hours) using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over promoters of ULBP1 and ASNS (active) and SAT2 satellite repeats, SAT Alpha (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous ATF4 protein at specific gene loci using Anti-ATF4 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-ATF4 Rabbit Polyclonal Antibody (Product # PA5-27576, 3 µg) on sheared chromatin from 2 million MDA-MB-231 cells treated with Cobalt chloride (150 µM for 24 hours) using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over promoters of ULBP1 and ASNS (active) and SAT2 satellite repeats, SAT Alpha (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
