Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- NB100-852 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-852, RRID:AB_2058748
- Product name
- Goat Polyclonal ATF4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_001666.2
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Cellular stress due to impairment of collagen prolyl hydroxylation complex is rescued by the chaperone 4-phenylbutyrate.
Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury.
Activation of the Integrated Stress Response and Metabolic Dysfunction in a Murine Model of Sleep Apnea.
Hyperglycemia-induced GLP-1R downregulation causes RPE cell apoptosis.
Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I.
Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I.
Besio R, Garibaldi N, Leoni L, Cipolla L, Sabbioneda S, Biggiogera M, Mottes M, Aglan M, Otaify GA, Temtamy SA, Rossi A, Forlino A
Disease models & mechanisms 2019 Jun 20;12(6)
Disease models & mechanisms 2019 Jun 20;12(6)
Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury.
Zhang W, Zhong W, Sun Q, Sun X, Zhou Z
Scientific reports 2018 Jan 11;8(1):408
Scientific reports 2018 Jan 11;8(1):408
Activation of the Integrated Stress Response and Metabolic Dysfunction in a Murine Model of Sleep Apnea.
Khalyfa A, Qiao Z, Gileles-Hillel A, Khalyfa AA, Akbarpour M, Popko B, Gozal D
American journal of respiratory cell and molecular biology 2017 Oct;57(4):477-486
American journal of respiratory cell and molecular biology 2017 Oct;57(4):477-486
Hyperglycemia-induced GLP-1R downregulation causes RPE cell apoptosis.
Kim DI, Park MJ, Choi JH, Lim SK, Choi HJ, Park SH
The international journal of biochemistry & cell biology 2015 Feb;59:41-51
The international journal of biochemistry & cell biology 2015 Feb;59:41-51
Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I.
Tsujimoto A, Nyunoya H, Morita T, Sato T, Shimotohno K
Journal of virology 1991 Mar;65(3):1420-6
Journal of virology 1991 Mar;65(3):1420-6
Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I.
Tsujimoto A, Nyunoya H, Morita T, Sato T, Shimotohno K
Journal of virology 1991 Mar;65(3):1420-6
Journal of virology 1991 Mar;65(3):1420-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF4 Antibody [NB100-852] - Raji cell lysate (RIPA buffer, 35 ug total protein). Primary antibody at 1 ug/ml, 1 hr incubation. ATF4 antibody detected using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF4 Antibody [NB100-852] - Lysate of cell line K562 (35 ug protein in RIPA buffer). Primary antibody at 0.3 ug/ml, 1 hr incubation. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF4 Antibody [NB100-852] - Ethanol feeding induced ATF4 expression in mouse liver which was suppressed by Lipin1 overexpression in adipose tissue. Western blot image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF4 Antibody [NB100-852] - Staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.