Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- ABIN394112 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (AA 171-271) antibody
- Antibody type
- Monoclonal
- Description
- This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 171-271
- Isotype
- IgG
- Antibody clone number
- 2B3
- Vial size
- 100 μg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Submitted references Oxidized phospholipids regulate expression of ATF4 and VEGF in endothelial cells via NRF2-dependent mechanism: novel point of convergence between electrophilic and unfolded protein stress pathways.
The GCN2-ATF4 pathway is critical for tumour cell survival and proliferation in response to nutrient deprivation.
How do tumours adapt to nutrient stress?
The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells.
Regulation of autophagy by ATF4 in response to severe hypoxia.
Afonyushkin T, Oskolkova OV, Philippova M, Resink TJ, Erne P, Binder BR, Bochkov VN
Arteriosclerosis, thrombosis, and vascular biology 2010 May;30(5):1007-13
Arteriosclerosis, thrombosis, and vascular biology 2010 May;30(5):1007-13
The GCN2-ATF4 pathway is critical for tumour cell survival and proliferation in response to nutrient deprivation.
Ye J, Kumanova M, Hart LS, Sloane K, Zhang H, De Panis DN, Bobrovnikova-Marjon E, Diehl JA, Ron D, Koumenis C
The EMBO journal 2010 Jun 16;29(12):2082-96
The EMBO journal 2010 Jun 16;29(12):2082-96
How do tumours adapt to nutrient stress?
Wek RC, Staschke KA
The EMBO journal 2010 Jun 16;29(12):1946-7
The EMBO journal 2010 Jun 16;29(12):1946-7
The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells.
Liew CW, Bochenski J, Kawamori D, Hu J, Leech CA, Wanic K, Malecki M, Warram JH, Qi L, Krolewski AS, Kulkarni RN
The Journal of clinical investigation 2010 Aug;120(8):2876-88
The Journal of clinical investigation 2010 Aug;120(8):2876-88
Regulation of autophagy by ATF4 in response to severe hypoxia.
Rzymski T, Milani M, Pike L, Buffa F, Mellor HR, Winchester L, Pires I, Hammond E, Ragoussis I, Harris AL
Oncogene 2010 Aug 5;29(31):4424-35
Oncogene 2010 Aug 5;29(31):4424-35
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Supportive validation
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- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ELISA
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC