Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00004724-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004724-M01, RRID:AB_540769
- Product name
- Mouse Monoclonal Ndufs4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NDUFS4 - NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi ( Cat # H00004724-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody (M01), clone 1A1 (Cat # H00004724-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.Lane 1: NDUFS4 transfected lysate(20.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - NDUFS4 monoclonal antibody (M01), clone 1A1 Analysis of NDUFS4 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Ndufs4 Antibody (1A1) [H00004724-M01] - Detection limit for recombinant GST tagged NDUFS4 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.