PAB3498

antibody from Abnova Corporation

Targeting: RIPK2 CARD3, CARDIAK, RICK, RIP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3498

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3498, RRID:AB_1580165

Product name

RIPK2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RIPK2.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.

Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research 2003 Oct;13(10):2265-70

---

Equilibrium and kinetic folding of an alpha-helical Greek key protein domain: caspase recruitment domain (CARD) of RICK.

Chen YR, Clark AC
Biochemistry 2003 May 27;42(20):6310-20

---

CARD6 is a modulator of NF-kappa B activation by Nod1- and Cardiak-mediated pathways.

Stehlik C, Hayashi H, Pio F, Godzik A, Reed JC
The Journal of biological chemistry 2003 Aug 22;278(34):31941-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of RIPK2 polyclonal antibody (Cat # PAB3498) in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with RIPK2 polyclonal antibody (Cat # PAB3498) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)