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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NBP1-97631 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97631, RRID:AB_11188372
- Product name
- Rat Monoclonal RIPK2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references PIDD mediates NF-kappaB activation in response to DNA damage.
Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity.
Janssens S, Tinel A, Lippens S, Tschopp J
Cell 2005 Dec 16;123(6):1079-92
Cell 2005 Dec 16;123(6):1079-92
Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity.
Lu C, Wang A, Dorsch M, Tian J, Nagashima K, Coyle AJ, Jaffee B, Ocain TD, Xu Y
The Journal of biological chemistry 2005 Apr 22;280(16):16278-83
The Journal of biological chemistry 2005 Apr 22;280(16):16278-83
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: RIPK2 Antibody (Nick-1) [NBP1-97631] - Detection of endogenous human RIP2 in different human cell lines by the MAb to RIP2 (Nick-1). Method: Total cell extracts (50ug) from Jurkat (lane1), THP-1 (lane 2) and HT1080 (lane 3) cell ljnes were run on a SDS-gel, and revealed with MAb to RIP2 (Nick-1) (1:1000 in 5% milk powder) for 1 hour at rooom temperature followed by incubation with HRP-coupled anti-rat secondary antibody (1:4000).
