Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS1983 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS1983, RRID:AB_1663737
- Product name
- Recoverin (D143) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 120-160 of Human Recoverin.
- Description
- Recoverin is implicated in the regulation of rhodopsin kinase activity that contributes to the adaptation to background illumination in retinal photoreceptor cells. Recoverin, a Ca2+-binding photoreceptor protein, is recognized as an autoantigen of cancer-associated retinopathy (CAR), which is a rare paraneoplastic neurological syndrome characterized by the degeneration of retinal photoreceptors and associated with small-cell lung cancer. Recoverin is a heterogeneously myristoylated protein that inhibits rhodopsin kinase by inhibiting its phosphorylation. Ca2+ is required for Recoverin to bind rhodopsin kinase. In addition, the binding of Recoverin-rhodopsin kinase is weakened by autophosphorylation of the kinase and is strengthened by the presence of ADP. Upon accommodating two Ca2+ ions, Recoverin extrudes a myristoyl group and associates with the lipid bilayer membrane.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of Recoverin (D143) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate