LS-C671734

antibody from LSBio

Targeting: KTN1 CG1, KIAA0004, KNT

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C671734

Provider

LSBio

Product name

KTN1 / Kinectin Antibody LS-C671734

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in MCF-7 whole cell lysate, Jurkat whole cell lysate. All lanes: KTN1 antibody at 3.4 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 157, 150, 151, 153 KDa. Observed band size: 157 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating KTN1 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of KTN1 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: KTN1 Antibody (6µg) + A549 whole cell lysate (1mg) Lane 3: A549 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating KTN1 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of KTN1 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: KTN1 Antibody (6µg) + A549 whole cell lysate (1mg) Lane 3: A549 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating KTN1 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of KTN1 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: KTN1 Antibody (6µg) + A549 whole cell lysate (1mg) Lane 3: A549 whole cell lysate (20µg)