PA5-116630

antibody from Invitrogen Antibodies

Targeting: POLRMT APOLMT, h-mtRPOL, MTRNAP, MTRPOL

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-116630

Provider

Invitrogen Antibodies

Product name

POLRMT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: HeLa, HepG2, 293T Immunogen sequence: SSASPQEQDQ DRRKDWGHVE LLEVLQARVR QLQAESVSEV VVNRVDVARL PECGSGDGSL QPPRKVQMGA KDATPVPCGR WAKILEKDKR TQQMRMQRLK AKLQMPFQSG EFKALTRRLQ VEPRLLSKQM AGCLEDCTRQ APESPWEEQL ARLLQEAPGK LSLDVEQAPS GQHSQAQLSG QQQRLLAFF

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.89 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of POLRMT in C6 cells using POLRMT Polyclonal Antibody (Product # PA5-116630) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of POLRMT in U-2 OS cells using POLRMT Polyclonal Antibody (Product # PA5-116630) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of POLRMT in C6 cells. Samples were incubated with POLRMT Polyclonal antibody (Product # PA5-116630) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of POLRMT in U-2 OS cells. Samples were incubated with POLRMT Polyclonal antibody (Product # PA5-116630) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of POLRMT in 200 μg extracts of HepG2 cells. Samples were precipitated with 3 μg POLRMT Polyclonal antibody (Product # PA5-116630). Western blot was performed from the immunoprecipitate using POLRMT Polyclonal antibody (Product # PA5-116630) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of POLRMT in 200 µg extracts of HepG2 cells using POLRMT Polyclonal Antibody (Product # PA5-116630). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of POLRMT in 200 µg extracts of HepG2 cells using POLRMT Polyclonal Antibody (Product # PA5-116630). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100