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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- A302-329A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GW182 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GW182 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody A302-329A (lot A302-329A-2) used for IP at 6 µg/mg lysate. GW182 was also immunoprecipitated by a previous lot (lot A302-329A-1) of this antibody. For blotting immunoprecipitated GW182, A302-329A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GW182 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GW182 antibody A302-329A (lot A302-329A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
