PA5-52172

antibody from Invitrogen Antibodies

Targeting: NDUFB5 CI-SGDH, MGC12314, SGDH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52172

Provider

Invitrogen Antibodies

Product name

NDUFB5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: EGYVPEHWEY YKHPISRWIA RNFYDSPEKI YERTMAVLQI EAEKAELRVK ELEVRKLMHV RGDGPWYYYE TIDKELIDHS PKATPDN Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 77%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NDUFB5 in Lane 1: Marker (kDa) 220, 112, 84, 47, 32, 26, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a NDUFB5 Polyclonal Antibody (Product # PA5-52172).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NDUFB5 in human cell line U-2 OS shows positivity in mitochondria & nucleus but excluded from the nucleoli. Samples were probed using a NDUFB5 Polyclonal Antibody (Product # PA5-52172).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NDUFB5 in human heart muscle and pancreas tissues using NDUFB5 Polyclonal Antibody (Product # PA5-52172). Corresponding NDUFB5 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NDUFB5 in human heart muscle using NDUFB5 Polyclonal Antibody (Product # PA5-52172) shows strong positivity in mitochondria in cardiomyocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NDUFB5 in human kidney using NDUFB5 Polyclonal Antibody (Product # PA5-52172) shows strong positivity in mitochondria in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NDUFB5 in human pancreas using NDUFB5 Polyclonal Antibody (Product # PA5-52172) shows weak positivity in mitochondria in exocrine glandular cells.