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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-109908 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LGR4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HLESLTLTGT KISSIPNNLC QEQKMLRTLD LSYNNIRDLP SFNGCHALEE ISLQRNQIYQ IKEGTFQGLI SLRILDLSRN LIHEIHSRAF ATLGPITNLD VSFNELTSFP TEGLNGLNQL KLVGNFKLKE ALAAKDFVNL RSLSVPYAYQ CCAFWGCDSY ANLNTEDNSL QDHSVAQEKG TADAANVTST LENEEHSQII IHCTPSTGAF KPCEYLLGSW M
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LGR4 in extracts of various cell lines. Samples were incubated with LGR4 Polyclonal antibody (Product # PA5-109908) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of LGR4 in human placenta. Samples were incubated with LGR4 Polyclonal antibody (Product # PA5-109908) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of LGR4 in human placenta. Samples were incubated with LGR4 Polyclonal antibody (Product # PA5-109908) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
