PA1-41062

antibody from Invitrogen Antibodies

Targeting: CCR1 CD191, CKR-1, CMKBR1, MIP1aR, SCYAR1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41062

Provider

Invitrogen Antibodies

Product name

CCR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: A375, antigen standard for CCR1 (transient overexpression lysate).

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Met-RANTES preserves the blood-brain barrier through inhibiting CCR1/SRC/Rac1 pathway after intracerebral hemorrhage in mice.

Yan J, Xu W, Lenahan C, Huang L, Ocak U, Wen J, Li G, He W, Le C, Zhang JH, Mo L, Tang J
Fluids and barriers of the CNS 2022 Jan 21;19(1):7

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Tumor-associated neutrophils suppress antitumor immunity of NK cells through the PD-L1/PD-1 axis.

Sun R, Xiong Y, Liu H, Gao C, Su L, Weng J, Yuan X, Zhang D, Feng J
Translational oncology 2020 Oct;13(10):100825

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CCR1 Activation Promotes Neuroinflammation Through CCR1/TPR1/ERK1/2 Signaling Pathway After Intracerebral Hemorrhage in Mice.

Yan J, Zuo G, Sherchan P, Huang L, Ocak U, Xu W, Travis ZD, Wang W, Zhang JH, Tang J
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2020 Jul;17(3):1170-1183

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Crosstalk between CCL7 and CCR3 promotes metastasis of colon cancer cells via ERK-JNK signaling pathways.

Lee YS, Kim SY, Song SJ, Hong HK, Lee Y, Oh BY, Lee WY, Cho YB
Oncotarget 2016 Jun 14;7(24):36842-36853

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Human heart lysate probed with a CD191/CCR1 polyclonal antibody (Product # PA1-41062) at 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Human heart lysate probed with a CD191/CCR1 polyclonal antibody (Product # PA1-41062) at 2 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded normal human spleen tissue stained with a CD191/CCR1 polyclonal antibody (Product # PA1-41062) (5 µg/mL), peroxidase-conjugate and DAB chromogen. Note granulocyte staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Image 1: Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and a CD191/CCR1 polyclonal antibody (Product # PA1-41062) with CY5 goat anti-rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 µL (blue histogram) and 10 µL (green histogram).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Temporal expressions of CCR1, CCL5, p-SRC, and Rac1 after ICH in mice. A Representative western blot images and quantitative analyses of time course of CCR1, CCL5, p-SRC, and Rac1 expressions within the ipsilateral hemisphere after ICH. Data were expressed as mean +- SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. sham group. n = 6/group. B The four black squares indicated perihematomal regions for microphotograph of the immunofluorescence staining. Double immunofluorescence staining for CCR1 (green) in endothelial cells (vWF, red), astrocytes (GFAP, red), and neurons (NeuN, red) within peri-hematoma area 24 h after ICH. Nuclei were stained with DAPI (blue). Scale bar = 50 mum. n = 2/group