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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA5-14711 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Follicle Stimulating Hormone Monoclonal Antibody (P2B4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-14711 targets Follicle Stimulating Hormone in ELISA, IP, and RIA applications and shows reactivity with Human samples. The MA5-14711 immunogen is human Follicle Stimulating Hormone. MA5-14711 detects Follicle Stimulating Hormone which has a predicted molecular weight of approximately 13 kDa. Product MA514711 is a smaller package size of MIF2706 (formerly sold as a Seradyn product).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- P2B4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Submitted references Downregulation of the GHRH/GH/IGF1 axis in a mouse model of Börjeson-Forssman-Lehman syndrome.
McRae HM, Eccles S, Whitehead L, Alexander WS, Gécz J, Thomas T, Voss AK
Development (Cambridge, England) 2020 Oct 23;147(21)
Development (Cambridge, England) 2020 Oct 23;147(21)
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Follicle Stimulating Hormone was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Follicle Stimulating Hormone (P2B4) Mouse Monoclonal Antibody (Product # MA5-14711) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
