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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MA5-14711 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Follicle Stimulating Hormone Monoclonal Antibody (P2B4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-14711 targets Follicle Stimulating Hormone in ELISA, IP, and RIA applications and shows reactivity with Human samples. The MA5-14711 immunogen is human Follicle Stimulating Hormone. MA5-14711 detects Follicle Stimulating Hormone which has a predicted molecular weight of approximately 13 kDa. Product MA514711 is a smaller package size of MIF2706 (formerly sold as a Seradyn product).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- P2B4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Submitted references Downregulation of the GHRH/GH/IGF1 axis in a mouse model of Börjeson-Forssman-Lehman syndrome.
McRae HM, Eccles S, Whitehead L, Alexander WS, Gécz J, Thomas T, Voss AK
Development (Cambridge, England) 2020 Oct 23;147(21)
Development (Cambridge, England) 2020 Oct 23;147(21)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on 30 µg of Mouse ovary tissue (Lane 1) and 10 µL of Human female plasma (Lane 2). The blots were probed with Follicle Stimulating Hormone Mouse Monoclonal antibody (Product # MA5-14711, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A ~ 21 kDa band corresponding to Follicle Stimulating Hormone was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001).The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on 30 µg of Mouse ovary tissue (Lane 1) and 10 µL of Human female plasma (Lane 2). The blots were probed with Follicle Stimulating Hormone Mouse Monoclonal antibody (Product # MA5-14711, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A ~ 21 kDa band corresponding to Follicle Stimulating Hormone was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001).The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Follicle Stimulating Hormone was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Follicle Stimulating Hormone (P2B4) Mouse Monoclonal Antibody (Product # MA5-14711) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
