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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-111750 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Follicle Stimulating Hormone Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: SH-SY5Y cell, rat pituitary tissue, human pituitary tissue, human liver tissue, mouse pituitary tissue, SH-SY5Y.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FSH beta in SH-SY5Y cell lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with FSH beta polyclonal antibody (Product # PA5-111750), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FSH beta in paraffin-embedded human pituitary tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with FSH beta polyclonal antibody (Product # PA5-111750) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FSH beta in paraffin-embedded rat pituitary tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with FSH beta polyclonal antibody (Product # PA5-111750) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FSH beta in paraffin-embedded mouse pituitary tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with FSH beta polyclonal antibody (Product # PA5-111750) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FSH beta in paraffin-embedded human liver tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with FSH beta polyclonal antibody (Product # PA5-111750) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FSH beta in SH-SY5Y cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with FSH beta polyclonal antibody (Product # PA5-111750) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:1000 (30 min).
