MA5-14695

antibody from Invitrogen Antibodies

Targeting: FSHB

ELISA

Immunoprecipitation

Radioimmunoassay

Antibody data

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Validation data

Product number: MA5-14695 - Provider product page
Provider: Invitrogen Antibodies
Product name: Follicle Stimulating Hormone Monoclonal Antibody (P4G2)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-14695 targets Follicle Stimulating Hormone in ELISA, IP, and RIA applications and shows reactivity with Human samples. The MA5-14695 immunogen is human Follicle Stimulating Hormone. MA5-14695 detects Follicle Stimulating Hormone which has a predicted molecular weight of approximately 13 kDa. Product MA514695 is a smaller package size of MIF2709 (formerly sold as a Seradyn product).
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: P4G2
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

FSHB protein structure - MA5-14695 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on 30 µg of Mouse ovary tissue (Lane 1) and 10 µL of Human female plasma (Lane 2). The blots were probed with Follicle Stimulating Hormone Mouse Monoclonal antibody (Product # MA5-14695, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A ~ 21 kDa band corresponding to Follicle Stimulating Hormone was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on 30 µg of Mouse ovary tissue (Lane 1) and 10 µL of Human female plasma (Lane 2). The blots were probed with Follicle Stimulating Hormone Mouse Monoclonal antibody (Product # MA5-14695, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A ~ 21 kDa band corresponding to Follicle Stimulating Hormone was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).