PA5-89979

antibody from Invitrogen Antibodies

Targeting: IPO8 IMP8, RANBP8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89979

Provider

Invitrogen Antibodies

Product name

Importin 8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: TRQLVNREDR SKAEKADMEE NEEISSDEEE TNVTAQAMQS NNGRGEDEEE EDDDWDEEVL EETALEGFST PLDLDNSVDE YQFFTQALIT VQSRDAAWYQ LLMAPLSEDQ RTALQEVYTL AEHRRTVAEA KKKIEQQGGF TFENKGVLSA FNFGTVPSNN; Positive Samples: HeLa, Jurkat, Mouse brain, Rat testis; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.01 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using IPO8 Polyclonal antibody (Product # PA5-89979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Importin 8 in extracts of various cell lines using Importin 8 Polyclonal Antibody (Product # PA5-89979) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Importin 8 was performed in L929 cells using Importin 8 Polyclonal Antibody (Product # PA5-89979) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Importin 8 was performed in C6 cells using Importin 8 Polyclonal Antibody (Product # PA5-89979) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Importin 8 was performed in HeLa cells using Importin 8 Polyclonal Antibody (Product # PA5-89979) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Importin 8 was performed in L929 cells using Importin 8 Polyclonal Antibody (Product # PA5-89979) at a dilution of 1:100. Blue: DAPI for nuclear staining.