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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [2]
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- Product number
- AF949 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse ADAM9 Ectodomain Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse ADAM9 Ectodomain in direct ELISAs and Western blots. In Western blots no cross-reactivity with the Ectodomain of recombinant mouse ADAM10 and rhADAM8, 15, and 17 (TACE) is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q61072- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A Disintegrin and Metalloproteinase 9 Domain (ADAM9) Is a Major Susceptibility Factor in the Early Stages of Encephalomyocarditis Virus Infection.
Sorting nexin 9 (SNX9) regulates levels of the transmembrane ADAM9 at the cell surface.
ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT.
Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis.
The association between laminin and microglial morphology in vitro.
The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein.
Accelerated wound repair in ADAM-9 knockout animals.
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
Bazzone LE, King M, MacKay CR, Kyawe PP, Meraner P, Lindstrom D, Rojas-Quintero J, Owen CA, Wang JP, Brass AL, Kurt-Jones EA, Finberg RW
mBio 2019 Feb 5;10(1)
mBio 2019 Feb 5;10(1)
Sorting nexin 9 (SNX9) regulates levels of the transmembrane ADAM9 at the cell surface.
Mygind KJ, Störiko T, Freiberg ML, Samsøe-Petersen J, Schwarz J, Andersen OM, Kveiborg M
The Journal of biological chemistry 2018 May 25;293(21):8077-8088
The Journal of biological chemistry 2018 May 25;293(21):8077-8088
ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT.
Lin CY, Cho CF, Bai ST, Liu JP, Kuo TT, Wang LJ, Lin YS, Lin CC, Lai LC, Lu TP, Hsieh CY, Chu CN, Cheng DC, Sher YP
Scientific reports 2017 Nov 8;7(1):15108
Scientific reports 2017 Nov 8;7(1):15108
Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis.
Giebeler N, Schönefuß A, Landsberg J, Tüting T, Mauch C, Zigrino P
Oncogene 2017 Aug 31;36(35):5058-5067
Oncogene 2017 Aug 31;36(35):5058-5067
The association between laminin and microglial morphology in vitro.
Tam WY, Au NP, Ma CH
Scientific reports 2016 Jun 23;6:28580
Scientific reports 2016 Jun 23;6:28580
The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein.
Delarasse C, Auger R, Gonnord P, Fontaine B, Kanellopoulos JM
The Journal of biological chemistry 2011 Jan 28;286(4):2596-606
The Journal of biological chemistry 2011 Jan 28;286(4):2596-606
Accelerated wound repair in ADAM-9 knockout animals.
Mauch C, Zamek J, Abety AN, Grimberg G, Fox JW, Zigrino P
The Journal of investigative dermatology 2010 Aug;130(8):2120-30
The Journal of investigative dermatology 2010 Aug;130(8):2120-30
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
The Journal of cell biology 2006 Oct 9;175(1):179-91
The Journal of cell biology 2006 Oct 9;175(1):179-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse ADAM9 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and WI-38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF949) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human ADAM9 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF949) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 78 and 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
