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Western blot
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- Antibody Data
- Antigen structure
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- Western blot [2]
- Flow cytometry [1]
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- Product number
- RQ6819 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ADAM9 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ADAM9 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ADAM9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat lung, 2) rat liver, 3) rat heart, 4) rat C6, 5) mouse lung, 6) mouse liver, 7) mouse heart and 8) mouse C2C12 cell lysate with ADAM9 antibody. Expected molecular weight: 90-100 kDa (pro-ADAM9-L form) and 70-80 kDa (ADMA9-L form).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) U-87 MG, 2) A431, 3) A549, 4) U-251, 5) U-2 OS, 6) HeLa, 7) HaCat and 8) SiHa cell lysate with ADAM9 antibody. Expected molecular weight: 90-100 kDa (pro-ADAM9-L form) and 70-80 kDa (ADMA9-L form).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-251 cells with ADAM9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAM9 antibody.
