PA5-25959

antibody from Invitrogen Antibodies

Targeting: ADAM9 CORD9, KIAA0021, MCMP, MDC9, Mltng

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25959

Provider

Invitrogen Antibodies

Product name

ADAM9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

An Integrative Data Mining and Omics-Based Translational Model for the Identification and Validation of Oncogenic Biomarkers of Pancreatic Cancer.

Long NP, Jung KH, Anh NH, Yan HH, Nghi TD, Park S, Yoon SJ, Min JE, Kim HM, Lim JH, Kim JM, Lim J, Lee S, Hong SS, Kwon SW
Cancers 2019 Jan 29;11(2)

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ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.

Li W, Wang D, Sun X, Zhang Y, Wang L, Suo J
International journal of molecular medicine 2019 Feb;43(2):914-926

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ADAM9 contributes to vascular invasion in pancreatic ductal adenocarcinoma.

Oria VO, Lopatta P, Schmitz T, Preca BT, Nyström A, Conrad C, Bartsch JW, Kulemann B, Hoeppner J, Maurer J, Bronsert P, Schilling O
Molecular oncology 2019 Feb;13(2):456-479

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ADAM9 in formalin-fixed and paraffin-embedded human lung carcinoma. Samples were incubated with ADAM9 polyclonal antibody (Product # PA5-25959) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MCF-7 cells using an ADAM9 polyclonal antibody (Product # PA5-25959) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Immunohistochemical analysis showing the expression of ADAM8, ADAM9, ADAM10, ADAM12 and ADAM17 in adjacent non-cancerous gastric tissues, gastric cancer tissues, positive metastatic lymph node tissues, and negative metastatic lymph node tissues. Scale bars, 50 u M. ADAM, disintegrin and metalloproteinase domain-containing protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Immunohistochemical analysis for determination of LAMC2 , ADAM9 , and ANXA2 ( Annexin A2 ) gene expression. ( a ) Scoring system for the three proteins in pancreatic tissues. ( b ) IHC scores of LAMC2 , ADAM9 , and ANXA2 in pancreatic tumors and normal controls ( n = 86). ***: p < 0.001; ****: p < 0.0001. ( c ) Pairwise correlation of LAMC2 , ADAM9 , and ANXA2 expression. ( d ) Pairwise correlation of LAMC2 , ADAM9 , and ANXA9 at gene expression level (log 2 of transcripts per million) in TGCA PC, TCGA normal pancreas, and Genotype-Tissue Expression Project (GTEx) derived normal pancreas.