Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4827 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IFITM1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IFITM1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human (rh) IFITM2 and rhIFITM3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.
Wu WL, Grotefend CR, Tsai MT, Wang YL, Radic V, Eoh H, Huang IC
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 3;114(27):7112-7117
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 3;114(27):7112-7117
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human IFITM1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, TF-1 human erythroleukemic cell line, human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFITM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.