12323-1-AP

antibody from Proteintech Group

Targeting: PAX6 AN, AN1, AN2, D11S812E, WAGR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

12323-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12323-1-AP, RRID:AB_2159695

Product name

PAX6 antibody

Antibody type

Polyclonal

Description

KD/KO validated PAX6 antibody (Cat. #12323-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Human neuropathology confirms projection neuron and interneuron defects and delayed oligodendrocyte production and maturation in FOXG1 syndrome.

Wilpert NM, Marguet F, Maillard C, Guimiot F, Martinovic J, Drunat S, Attié-Bitach T, Razavi F, Tessier A, Capri Y, Laquerrière A, Bahi-Buisson N
European journal of medical genetics 2021 Sep;64(9):104282

---

Immunohistochemical evidence for adult human neurogenesis in health and disease.

Gault N, Szele FG
WIREs mechanisms of disease 2021 Nov;13(6):e1526

---

Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.

Guo X, Cui C, Song J, He Q, Zang N, Hu H, Wang X, Li D, Wang C, Hou X, Li X, Liang K, Yan F, Chen L
Molecular and cellular endocrinology 2021 Nov 1;537:111425

---

Perinatal exposure to nonylphenol promotes proliferation of granule cell precursors in offspring cerebellum: Involvement of the activation of Notch2 signaling.

Fang Y, Che X, You M, Xu Y, Wang Y
Neurochemistry international 2020 Nov;140:104843

---

PAX6 contributes to the activation and proliferation of hepatic stellate cells via activating Hedgehog/GLI1 pathway.

Li C, Tan YH, Sun J, Deng FM, Liu YL
Biochemical and biophysical research communications 2020 May 28;526(2):314-320

---

Δ⁹-tetrahydrocannabinol and 2-AG decreases neurite outgrowth and differentially affects ERK1/2 and Akt signaling in hiPSC-derived cortical neurons.

Shum C, Dutan L, Annuario E, Warre-Cornish K, Taylor SE, Taylor RD, Andreae LC, Buckley NJ, Price J, Bhattacharyya S, Srivastava DP
Molecular and cellular neurosciences 2020 Mar;103:103463

---

Computational and functional analyses of T2D GWAS SNPs for transcription factor binding.

Cheng M, Huang X, Zhang M, Huang Q
Biochemical and biophysical research communications 2020 Mar 12;523(3):658-665

---

Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea.

Díaz NM, Lang RA, Van Gelder RN, Buhr ED
Investigative ophthalmology & visual science 2020 Jun 3;61(6):37

---

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.

Sun X, Peng X, Cao Y, Zhou Y, Sun Y
Nature communications 2020 Jun 12;11(1):2984

---

Zinc Promotes Patient-Derived Induced Pluripotent Stem Cell Neural Differentiation via ERK-STAT Signaling.

Yang M, Bao D, Shi A, Yuan H, Wang J, He W, Tong X, Qin H
Stem cells and development 2020 Jul 1;29(13):863-875

---

The generation of an induced pluripotent stem cell line (DCGi001-A) from an individual with FOXG1 syndrome carrying the c.460dupG (p.Glu154fs) variation in the FOXG1 gene.

Waite AJ, Millar D, Clarke A
Stem cell research 2020 Dec;49:102018

---

Down-regulation of ATF1 leads to early neuroectoderm differentiation of human embryonic stem cells by increasing the expression level of SOX2.

Yang SC, Liu JJ, Wang CK, Lin YT, Tsai SY, Chen WJ, Huang WK, Tu PA, Lin YC, Chang CF, Cheng CL, Lin H, Lai CY, Lin CY, Lee YH, Chiu YC, Hsu CC, Hsu SC, Hsiao M, Schuyler SC, Lu FL, Lu J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Sep;33(9):10577-10592

---

Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.

Huang M, Tailor J, Zhen Q, Gillmor AH, Miller ML, Weishaupt H, Chen J, Zheng T, Nash EK, McHenry LK, An Z, Ye F, Takashima Y, Clarke J, Ayetey H, Cavalli FMG, Luu B, Moriarity BS, Ilkhanizadeh S, Chavez L, Yu C, Kurian KM, Magnaldo T, Sevenet N, Koch P, Pollard SM, Dirks P, Snyder MP, Largaespada DA, Cho YJ, Phillips JJ, Swartling FJ, Morrissy AS, Kool M, Pfister SM, Taylor MD, Smith A, Weiss WA
Cell stem cell 2019 Sep 5;25(3):433-446.e7

---

Evolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex.

Arai Y, Cwetsch AW, Coppola E, Cipriani S, Nishihara H, Kanki H, Saillour Y, Freret-Hodara B, Dutriaux A, Okada N, Okano H, Dehay C, Nardelli J, Gressens P, Shimogori T, D'Onofrio G, Pierani A
Cell reports 2019 Oct 15;29(3):645-658.e5

---

LIMK1 and LIMK2 regulate cortical development through affecting neural progenitor cell proliferation and migration.

Mao R, Deng R, Wei Y, Han L, Meng Y, Xie W, Jia Z
Molecular brain 2019 Jul 18;12(1):67

---

Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.

Zhang J, Chen Y, Gao M, Wang Z, Liu R, Xia T, Liu S
ACS nano 2019 Feb 26;13(2):2050-2061

---

The PAX6-ZEB2 axis promotes metastasis and cisplatin resistance in non-small cell lung cancer through PI3K/AKT signaling.

Wu DM, Zhang T, Liu YB, Deng SH, Han R, Liu T, Li J, Xu Y
Cell death & disease 2019 Apr 25;10(5):349

---

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Zhong X, Cui P, Cai Y, Wang L, He X, Long P, Lu K, Yan R, Zhang Y, Pan X, Zhao X, Li W, Zhang H, Zhou Q, Gao P
Cell metabolism 2019 Apr 2;29(4):979-992.e4

---

Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells.

Li Z, Duan H, Li W, Hu X, Jia Y, Zhao C, Zhang S, Zhou Q, Shi W
Stem cells and development 2019 Apr 1;28(7):454-463

---

Hippocampal Radial Glial Subtypes and Their Neurogenic Potential in Human Fetuses and Healthy and Alzheimer's Disease Adults.

Cipriani S, Ferrer I, Aronica E, Kovacs GG, Verney C, Nardelli J, Khung S, Delezoide AL, Milenkovic I, Rasika S, Manivet P, Benifla JL, Deriot N, Gressens P, Adle-Biassette H
Cerebral cortex (New York, N.Y. : 1991) 2018 Jul 1;28(7):2458-2478

---

Hydrocephalus due to multiple ependymal malformations is caused by mutations in the MPDZ gene.

Saugier-Veber P, Marguet F, Lecoquierre F, Adle-Biassette H, Guimiot F, Cipriani S, Patrier S, Brasseur-Daudruy M, Goldenberg A, Layet V, Capri Y, Gérard M, Frébourg T, Laquerrière A
Acta neuropathologica communications 2017 May 1;5(1):36

---

Dynamic Expression Patterns of Progenitor and Neuron Layer Markers in the Developing Human Dentate Gyrus and Fimbria.

Cipriani S, Journiac N, Nardelli J, Verney C, Delezoide AL, Guimiot F, Gressens P, Adle-Biassette H
Cerebral cortex (New York, N.Y. : 1991) 2017 Jan 1;27(1):358-372

---

miR-342-5p Regulates Neural Stem Cell Proliferation and Differentiation Downstream to Notch Signaling in Mice.

Gao F, Zhang YF, Zhang ZP, Fu LA, Cao XL, Zhang YZ, Guo CJ, Yan XC, Yang QC, Hu YY, Zhao XH, Wang YZ, Wu SX, Ju G, Zheng MH, Han H
Stem cell reports 2017 Apr 11;8(4):1032-1045

---

Dynamic Expression Patterns of Progenitor and Pyramidal Neuron Layer Markers in the Developing Human Hippocampus.

Cipriani S, Nardelli J, Verney C, Delezoide AL, Guimiot F, Gressens P, Adle-Biassette H
Cerebral cortex (New York, N.Y. : 1991) 2016 Mar;26(3):1255-71

---

Olig2 regulates Purkinje cell generation in the early developing mouse cerebellum.

Ju J, Liu Q, Zhang Y, Liu Y, Jiang M, Zhang L, He X, Peng C, Zheng T, Lu QR, Li H
Scientific reports 2016 Jul 29;6:30711

---

MicroRNA-mediated non-cell-autonomous regulation of cortical radial glial transformation revealed by a Dicer1 knockout mouse model.

Zhang C, Ge X, Liu Q, Jiang M, Li MW, Li H
Glia 2015 May;63(5):860-76

---

PAK1 regulates cortical development via promoting neuronal migration and progenitor cell proliferation.

Pan X, Chang X, Leung C, Zhou Z, Cao F, Xie W, Jia Z
Molecular brain 2015 Jun 5;8:36

---

Abnormal cerebellar development and Purkinje cell defects in Lgl1-Pax2 conditional knockout mice.

Hou C, Ding L, Zhang J, Jin Y, Sun C, Li Z, Sun X, Zhang T, Zhang A, Li H, Gao J
Developmental biology 2014 Nov 1;395(1):167-81

---

A robust method to derive functional neural crest cells from human pluripotent stem cells.

Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR
American journal of stem cells 2013;2(2):119-31

---

Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a.

Zhao X, Wu J, Zheng M, Gao F, Ju G
Molecular biology of the cell 2012 Aug;23(15):2867-78

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human stomach tissue were subjected to SDS PAGE followed by western blot with 12323-1-AP(PAX6 antibody) at dilution of 1:300

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 12323-1-AP(PAX6 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The PAX6 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PAX6. This antibody recognizes human, mouse, rat antigen. The PAX6 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

12323-1-AP.pdf

Show more