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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 14-9914-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAX6 Monoclonal Antibody (AD2.38), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This AD2.38 monoclonal antibody reacts with human, mouse, and rat Pax6. This 47-kDa transcription factor is expressed during embryonic neurogenesis in the brain and central nervous system, as well as in neuroepithelial cells of the retina. Pax6 is involved in development of the eyes, nose, central nervous system, pancreas, and pituitary gland. Studies also demonstrate that Pax6 is crucial for alpha-cell differentiation and glucagon biosynthesis in the pancreas. Mutation of Pax6 results in eye disorders such as aniridia in humans and Small eye in mice. Applications Reported: This AD2.38 antibody has been reported for use in immunoblotting (WB) and immunocytochemistry. Applications Tested: This AD2.38 antibody has been tested by immunocytochemistry of fixed and permeabilized MV+ cells. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AD2.38
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Substantial somatic genomic variation and selection for BCOR mutations in human induced pluripotent stem cells.
Formation of three-dimensional cell aggregates expressing lens-specific proteins in various cultures of human iris-derived tissue cells and iPS cells.
Concise review: Pax6 transcription factor contributes to both embryonic and adult neurogenesis as a multifunctional regulator.
Role of Pax6 in development of the cerebellar system.
Rouhani FJ, Zou X, Danecek P, Badja C, Amarante TD, Koh G, Wu Q, Memari Y, Durbin R, Martincorena I, Bassett AR, Gaffney D, Nik-Zainal S
Nature genetics 2022 Sep;54(9):1406-1416
Nature genetics 2022 Sep;54(9):1406-1416
Formation of three-dimensional cell aggregates expressing lens-specific proteins in various cultures of human iris-derived tissue cells and iPS cells.
Hiramatsu N, Yamamoto N, Kato Y, Nagai N, Isogai S, Imaizumi K
Experimental and therapeutic medicine 2022 Aug;24(2):539
Experimental and therapeutic medicine 2022 Aug;24(2):539
Concise review: Pax6 transcription factor contributes to both embryonic and adult neurogenesis as a multifunctional regulator.
Osumi N, Shinohara H, Numayama-Tsuruta K, Maekawa M
Stem cells (Dayton, Ohio) 2008 Jul;26(7):1663-72
Stem cells (Dayton, Ohio) 2008 Jul;26(7):1663-72
Role of Pax6 in development of the cerebellar system.
Engelkamp D, Rashbass P, Seawright A, van Heyningen V
Development (Cambridge, England) 1999 Aug;126(16):3585-96
Development (Cambridge, England) 1999 Aug;126(16):3585-96
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized MV+ cells using 5 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 11-4714-42) (left) or Anti-Pax6 Purified (right) followed by Anti-Mouse TRITC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunostained specimens from each of the four steps in the Ex.1 method. The images show DAPI staining in the same area as that stained for PAX6, SOX2, and alphaA-crystallin in STEP-0, -1, -2, and -3. Scale bar, 100 um. Ex, experiment.
