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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-25970 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAX6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.
Sidney LE, Branch MJ, Dua HS, Hopkinson A
Cytotherapy 2015 Dec;17(12):1706-22
Cytotherapy 2015 Dec;17(12):1706-22
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PAX6 (arrow) using a PAX6 polyclonal antibody (Product # PA5-25970) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PAX6 polyclonal antibody (Product # PA5-25970) in Y79 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a PAX6 polyclonal antibody (Product # PA5-25970) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human brain tissue using a PAX6 polyclonal antibody (Product # PA5-25970), followed by HRP-conjugated secondary antibody and DAB staining.
