Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF596 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-21R Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-21 R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human IL-21 R is observed, and less than 1% cross-reactivity will recombinant mouse IL-2 R beta is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis.
Zhang X, Kiapour N, Kapoor S, Khan T, Thamilarasan M, Tao Y, Cohen S, Miller R, Sobel RA, Markovic-Plese S
Journal of immunology (Baltimore, Md. : 1950) 2019 Sep 1;203(5):1142-1150
Journal of immunology (Baltimore, Md. : 1950) 2019 Sep 1;203(5):1142-1150
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-21 R in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL-21 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF596, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).