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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PA5-37612 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-IRF3 (Ser386) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is 293 or COLO cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Granzyme B Induces IRF-3 Phosphorylation through a Perforin-Independent Proteolysis-Dependent Signaling Cascade without Inducing Cell Death.
Gapud EJ, Trejo-Zambrano MI, Gomez-Banuelos E, Tiniakou E, Antiochos B, Granville DJ, Andrade F, Casciola-Rosen L, Rosen A
Journal of immunology (Baltimore, Md. : 1950) 2021 Jan 15;206(2):335-344
Journal of immunology (Baltimore, Md. : 1950) 2021 Jan 15;206(2):335-344
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of extracts from 293 cells (Lane 2) and COLO cells (Lane 3) using IRF-3 (pSer386) polyclonal antibody (Product # PA5-37612) compared to the same antibody preincubated with a blocking peptide (Lane 1).
