Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX130422 - Provider product page
- Provider
- GeneTex
- Product name
- IRF3 (phospho Ser386) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation.
Soonthornvacharin S, Rodriguez-Frandsen A, Zhou Y, Galvez F, Huffmaster NJ, Tripathi S, Balasubramaniam VR, Inoue A, de Castro E, Moulton H, Stein DA, Sánchez-Aparicio MT, De Jesus PD, Nguyen Q, König R, Krogan NJ, García-Sastre A, Yoh SM, Chanda SK
Nature microbiology 2017 Mar 1;2:17022
Nature microbiology 2017 Mar 1;2:17022
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- IRF3 (phospho Ser386) antibody detects IRF3 (phospho Ser386) protein by western blot analysis. Un-treated (-) and treated (+, CIP treatment for 1hr) HeLa whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF3 (phospho Ser386) antibody (GTX130422) at a dilution of 1:5000.