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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003661-M10 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003661-M10, RRID:AB_1237756
- Product name
- IRF3 monoclonal antibody (M10), clone 3C8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant IRF3.
- Antigen sequence
MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFR
IPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDK
PDLPTWKRNFRSALSRKEGLRLAEDRSKDPHDPHK
IYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDIL
DELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPS
LDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFY
RGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTL
PDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQW
LWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKGK
EGGVFDLGPFIVGSWAPRSDYLHGRKRTLTTLCPL
VLCGGVMAPGPAVDQEARDGQGCAHVPQGLGRNGP
GRGCLLPGEYCGPAHFQQPPTLPHLRPVQGLPAGL
GGGHGFPGPWGDLSPRSSWCASNPPVPHHLNQ- Isotype
- IgG
- Antibody clone number
- 3C8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of IRF3 expression in transfected 293T cell line by IRF3 monoclonal antibody (M10), clone 3C8.Lane 1: IRF3 transfected lysate(47.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged IRF3 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to IRF3 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of IRF3 transfected lysate using anti-IRF3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IRF3 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
