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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500476 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500476, RRID:AB_2126996
- Product name
- IRF3 mouse monoclonal antibody, clone OTI4D4 (formerly 4D4)
- Antibody type
- Monoclonal
- Description
- IRF3 mouse monoclonal antibody, clone OTI4D4 (formerly 4D4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IRF3
- Isotype
- IgG
- Antibody clone number
- OTI4D4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-IRF3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (RC209951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-IRF3 mouse monoclonal antibody (TA500476) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF3(RC209951).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500476)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209951 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IRF3 antibody(TA500476), and then analyzed by flow cytometry.
- Validation comment
- FC
