Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-87506 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ITFTEGSGRS PRYALWFCVG ESWPQDQPWT KRLVMVKVVP TCLRALVEMA RVGGASSLEN TVDLHISNSH PLSLTSDQYK AYLQDLVEGM DFQGPGES
- Concentration
- 0.93 mg/mL
Submitted references Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.
von Roemeling CA, Wang Y, Qie Y, Yuan H, Zhao H, Liu X, Yang Z, Yang M, Deng W, Bruno KA, Chan CK, Lee AS, Rosenfeld SS, Yun K, Johnson AJ, Mitchell DA, Jiang W, Kim BYS
Nature communications 2020 Mar 20;11(1):1508
Nature communications 2020 Mar 20;11(1):1508
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using IRF3 Polyclonal antibody (Product # PA5-87506) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IRF3 in extracts of various cell lines using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using IRF3 Polyclonal antibody (Product # PA5-87506) at dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRF3 in paraffin-embedded human gastric cancer using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRF3 in paraffin-embedded human breast using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRF3 in paraffin-embedded human stomach using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRF3 in paraffin-embedded human gastric cancer using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 cGAS-STING pathway is essential for TMZ and anti-CD47 induced immune response. a Combination TMZ and anti-CD47 antibody treatment against murine GBM cells (GL261) increased the production of type I interferons in APCs. IFNA interferon alpha , IFNB interferon beta . n = 5, ** p < 0.01, one-side ANOVA with Bonferroni post hoc correction. b Western blot showing combined TMZ and anti-CD47 antibody treatment resulted in the activation of cGAS-STING cytoplasmic DNA sensing pathways in BM APCs. c Combination TMZ and anti-CD47 antibody treatment promoted p65 expression and nuclear translocation in BM APCs that is dependent on STING. d STING activation is critical to the increased the production of NF-kappaB cytokine TNFalpha and IL1beta in APCs in the setting of combined TMZ and anti-CD47 antibody treatment. n = 6, ** p < 0.01, unpaired Student''s t test. e Immunofluorescence staining of GL261 tumors implanted in WT and STING KO animals. Nuclear p-IRF3 (Ser 396) in CD45 + cells is indicative of STING signaling activation. f , g Quantification of CD45 + cell infiltration and percentage of p-IRF3 positive CD45 + , C57BL/6, n = 7; knockout, n = 6. Unpaired Student''s t test. h GL261 tumor volume in C57BL/6 or STING KO mice at day 20 following control or combination TMZ + aCD47 treatment, n = 5/group. i T-cell infiltration in GL261 tumors in C57BL/6 or STING KO mice at day 20 following control or combination TMZ + aCD47 treatment measured by CD3 + cells in total DAPI count per fie
