PA5-87506

antibody from Invitrogen Antibodies

Targeting: IRF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87506

Provider

Invitrogen Antibodies

Product name

IRF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: 293T, HeLa, Jurkat, HepG2, Mouse liver; Cellular Location: Cytoplasm, Nucleus Immunogen sequence: EVPKDKEGGV FDLGPFIVDL ITFTEGSGRS PRYALWFCVG ESWPQDQPWT KRLVMVKVVP TCLRALVEMA RVGGASSLEN TVDLHISNSH PLSLTSDQYK A

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.93 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

von Roemeling CA, Wang Y, Qie Y, Yuan H, Zhao H, Liu X, Yang Z, Yang M, Deng W, Bruno KA, Chan CK, Lee AS, Rosenfeld SS, Yun K, Johnson AJ, Mitchell DA, Jiang W, Kim BYS
Nature communications 2020 Mar 20;11(1):1508

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using IRF3 Polyclonal antibody (Product # PA5-87506) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IRF3 in extracts of various cell lines using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using IRF3 Polyclonal antibody (Product # PA5-87506) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IRF3 in paraffin-embedded human gastric cancer using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IRF3 in paraffin-embedded human breast using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IRF3 in paraffin-embedded human stomach using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IRF3 in paraffin-embedded human gastric cancer using IRF3 Polyclonal Antibody (Product # PA5-87506) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 cGAS-STING pathway is essential for TMZ and anti-CD47 induced immune response. a Combination TMZ and anti-CD47 antibody treatment against murine GBM cells (GL261) increased the production of type I interferons in APCs. IFNA interferon alpha , IFNB interferon beta . n = 5, ** p < 0.01, one-side ANOVA with Bonferroni post hoc correction. b Western blot showing combined TMZ and anti-CD47 antibody treatment resulted in the activation of cGAS-STING cytoplasmic DNA sensing pathways in BM APCs. c Combination TMZ and anti-CD47 antibody treatment promoted p65 expression and nuclear translocation in BM APCs that is dependent on STING. d STING activation is critical to the increased the production of NF-kappaB cytokine TNFalpha and IL1beta in APCs in the setting of combined TMZ and anti-CD47 antibody treatment. n = 6, ** p < 0.01, unpaired Student''s t test. e Immunofluorescence staining of GL261 tumors implanted in WT and STING KO animals. Nuclear p-IRF3 (Ser 396) in CD45 + cells is indicative of STING signaling activation. f , g Quantification of CD45 + cell infiltration and percentage of p-IRF3 positive CD45 + , C57BL/6, n = 7; knockout, n = 6. Unpaired Student''s t test. h GL261 tumor volume in C57BL/6 or STING KO mice at day 20 following control or combination TMZ + aCD47 treatment, n = 5/group. i T-cell infiltration in GL261 tumors in C57BL/6 or STING KO mice at day 20 following control or combination TMZ + aCD47 treatment measured by CD3 + cells in total DAPI count per fie