Explore
Validate
LearnPA5-88077
antibody from Invitrogen Antibodies
Targeting: IRF3
Western blot
ELISA
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-88077 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PHDPHKIYEF VNSGVGDFSQ PDTSPDTNGG GSTSDTQEDI LDELLGNMVL APLPDPGPPS LAVAPEPCPQ PLRSPSLDNP TPFPNLGPSE NPLKRLLVPG E Positive Samples: 293T, HeLa, Jurkat, HepG2; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.04 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of IRF3 in HeLa cells. Samples were incubated with IRF3 Polyclonal antibody (Product # PA5-88077) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of IRF3 in 300 μg extracts of 293T cells. Samples were precipitated with 3 μg IRF3 Polyclonal antibody (Product # PA5-88077). Western blot was performed from the immunoprecipitate using IRF3 Polyclonal antibody (Product # PA5-88077) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRF3 in paraffin-embedded human lung cancer. Samples were incubated with IRF3 Polyclonal antibody (Product # PA5-88077) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRF3 in paraffin-embedded human esophageal cancer. Samples were incubated with IRF3 Polyclonal antibody (Product # PA5-88077) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of IRF3 in HCT116 cell line using IRF3 Polyclonal Antibody (Product # PA5-88077) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of IRF3 in extracts of HCT116 cell line. Samples were incubated with IRF3 Polyclonal antibody (Product # PA5-88077) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
