F54481

antibody from NSJ Bioreagents

Targeting: IRF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54481

Provider

NSJ Bioreagents

Product name

IRF3 Antibody

Antibody type

Monoclonal

Description

This highly specific IRF3 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence applications with human, monkey samples.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

1522CT766.58.24

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the IRF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) monkey COS-7 and human 2) Daudi, 3) HepG2, 4) HT-29, 5) Jurkat and 6) MCF7 cell lysate with IRF3 antibody. Predicted molecular weight ~47 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human HeLa cells with IRF3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human kidney tissue with IRF3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human Jurkat cells with IRF3 antibody; Blue=isotype control, Green= IRF3 antibody.