11312-1-AP
antibody from Invitrogen Antibodies
Targeting: IRF3
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
- Other assay [1]
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Validation data
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- Product number
- 11312-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGTPKPRIL PWLVSQLDLG QLEGVAWVNK SRTRFRIPWK HGLRQDAQQE DFGIFQAWAE ATGAYVPGRD KPDLPTWKRN FRSALNRKEG LRLAEDRSKD PHDPHKIYEF VNSGVGDFSQ PDTSPDTNGG GSTSDTQEDI LDELLGNMVL APLPDPGPPS LAVAPEPCPQ PLRSPSLDNP TPFPNLGPSE NPLKRLLVPG EEWEFEVTAF YRGRQVFQQT ISCPEGLRLV GSEVGDRTLP GWPVTLPDPG MSLTDRGVMS YVRHVLSCLG GGLALWRAGQ WLWAQRLGHC HTYWAVSEEL LPNSGHGPDG EVPKDKEGGV FDLGPFIVGS WAPRSDYLHG RKRTLTTLCP LVLCGGVMAP GPAVDQEARD GQGCAHVPQG LGRNGPGRGC LLPGEYCGPA HFQQPPTLPH LRPVQGLPAG LGGGHGFPGP WGDLSPRSSW CASNPPVPHH LNQ (1-452 aa encoded by BC009395)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U-937 cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of IRF3 specific antibody (11312-1-AP, 1:5000) with si-control and si-IRF3 transfected Jurkat cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using IRF3 antibody 11312-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human IRF3 (Product # 11312-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with .2ug IRF3 antibody (11312-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IRF3 (IP:11312-1-AP, 3ug; Detection:11312-1-AP 1:1000) with Jurkat cells lysate 2400ug.