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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 648702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#648702, RRID:AB_2126988
- Product name
- Purified anti-IRF-3
- Antibody type
- Monoclonal
- Antigen
- Recombinant GST-IRF-3(56-427) protein
- Description
- IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Recent research indicates that it plays a critical role in stimulated TLR-mediated IL-27 P28 gene expression.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4 °C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-human IRF-3 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
