PA5-20087

Antibody data

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Validation data

Product number: PA5-20087
Provider: Invitrogen Antibodies
Product name: Anti-IRF3 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-20087
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is U937 cell lysate. PA5-20087 can be used with blocking peptide PEP-0205.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Provider	Type	Product Number
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of U937 whole cell lysate using a IRF3 polyclonal antibody (Product # PA5-20087) at (A) 1, (B) 2, and (C) 4 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of IRF3 was achieved by transfecting A-431 cells with IRF3 specific siRNAs (Silencer® select Product # s7509, s7507). Western blot analysis (Fig. a) was performed using membrane extracts from the IRF3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with IRF3 Polyclonal Antibody (Product # PA5-20087, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to IRF3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), THP-1 (Lane 2), HL-60 (Lane 3), U-937 (Lane 4), A549 (Lane 5), A-431 (Lane 6), MCF7 (Lane 7) and HT-29 (Lane 8). The blot was probed with Anti-IRF3 Polyclonal Antibody (Product # PA5-20087, 4 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). 50kDa and 33kDa bands corresponding to IRF3 isoforms were observed across all the cell lines tested. An uncharacterized band was observed at ~80kDa in certain cell lines.