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- Antibody Data
- Antigen structure
- References [5]
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- Immunohistochemistry [1]
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- Product number
- AF2299 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Crossveinless-2/CV-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Crossveinless-2/CV-2 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human CV-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAH66153- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references BMPER-induced BMP signaling promotes coronary artery remodeling.
Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
Development of the vertebral morphogenetic field in the mouse: interactions between Crossveinless-2 and Twisted Gastrulation.
Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
BMPER is a conserved regulator of hematopoietic and vascular development in zebrafish.
Dyer L, Wu Y, Moser M, Patterson C
Developmental biology 2014 Feb 15;386(2):385-94
Developmental biology 2014 Feb 15;386(2):385-94
Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
Zakin L, Chang EY, Plouhinec JL, De Robertis EM
Developmental biology 2010 Nov 1;347(1):204-15
Developmental biology 2010 Nov 1;347(1):204-15
Development of the vertebral morphogenetic field in the mouse: interactions between Crossveinless-2 and Twisted Gastrulation.
Zakin L, Metzinger CA, Chang EY, Coffinier C, De Robertis EM
Developmental biology 2008 Nov 1;323(1):6-18
Developmental biology 2008 Nov 1;323(1):6-18
Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM
Developmental cell 2008 Aug;15(2):248-60
Developmental cell 2008 Aug;15(2):248-60
BMPER is a conserved regulator of hematopoietic and vascular development in zebrafish.
Moser M, Yu Q, Bode C, Xiong JW, Patterson C
Journal of molecular and cellular cardiology 2007 Sep;43(3):243-53
Journal of molecular and cellular cardiology 2007 Sep;43(3):243-53
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Crossveinless-2/CV-2 in Mouse Embryo. Crossveinless-2/CV-2 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Mouse Crossveinless-2/CV-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2299) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the epithelium surrounding the nasal cavity. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
