60070-1-Ig

antibody from Proteintech Group

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Provider product page for 60070-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [32]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [2]

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Product number: 60070-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#60070-1-Ig, RRID:AB_2280158
Product name: IL-18 antibody
Antibody type: Monoclonal
Description: KD/KO validated IL-18 antibody (Cat. #60070-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, hamster and has been validated for the following applications: IHC, WB,ELISA.
Reactivity: Human, Hamster
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 5C6F8
Vial size: 20ul, 150ul

IL18 protein structure - 60070-1-Ig shown in red.

Submitted references miR-328-3p targets TLR2 to ameliorate oxygen-glucose deprivation injury and neutrophil extracellular trap formation in HUVECs via inhibition of the NF-κB signaling pathway.

Yao M, Fang C, Wang Z, Guo T, Wu D, Ma J, Wu J, Mo J
PloS one 2024;19(2):e0299382

Influence of SLC40A1 on Cytokine Interactions and Immune Infiltration in Glioblastoma.

Jiang J, Duan R, Zhu J, Yan J, Ye J, Luo C
Neuromolecular medicine 2024 May 16;26(1):21

Mechanistic study of Huangqi Guizhi Wuwu decoction amelioration of doxorubicin-induced cardiotoxicity by reducing oxidative stress and inhibiting cellular pyroptosis.

Chen Y, Xu M, Liu XM, Wang JX, Sun MF, Song JX, Guan P, Ji ES, Wang N
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Jun;175:116653

Overexpression of SESN1 improves mitochondrial damage and mitophagy, a potential therapeutic strategy for cognitive dysfunction after anaesthesia.

Sun L, Hong X, Wang D, Li Y
The European journal of neuroscience 2024 Jan;59(2):208-219

Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and reperfusion--From the perspective of glial-vascular unit.

Zhou C, Li JX, Zheng CX, Zhou XQ, Chen C, Qiu SW, Liu WH, Li H
Journal of ethnopharmacology 2024 Jan 10;318(Pt B):116990

Lactoferrin ameliorated obesity-induced endothelial dysfunction by inhibiting the Tak1/IL-18/eNOS pathway between PVAT and vascular endothelium.

Chen C, Yan Y, Wu Y, Lu M, Xing Y, Bai Y, Zhao H, Ding L, Wu Y, Xu J, Qin L, Lv H, Zhang Z
Free radical biology & medicine 2024 Feb 20;212:309-321

Mesenchymal Stem Cells-Derived Exosomes Alleviate Acute Lung Injury by Inhibiting Alveolar Macrophage Pyroptosis.

Liu P, Yang S, Shao X, Li C, Wang Z, Dai H, Wang C
Stem cells translational medicine 2024 Apr 15;13(4):371-386

Transcutaneous electrical acupoint stimulation alleviates cerebral ischemic injury through the TLR4/MyD88/NF-κ B pathway.

Wu L, Tan Z, Su L, Dong F, Xu G, Zhang F
Frontiers in cellular neuroscience 2023;17:1343842

PACS-2 deficiency aggravates tubular injury in diabetic kidney disease by inhibiting ER-phagy.

Yang J, Li L, Li C, Chen W, Liu Y, Luo S, Zhao C, Han Y, Yang M, Zhao H, Jiang N, Xi Y, Tang C, Cai J, Xiao L, Liu H, Sun L
Cell death & disease 2023 Oct 4;14(10):649

Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing Exosomal miR-182-5p.

Li X, Cao Y, Xu X, Wang C, Ni Q, Lv X, Yang C, Zhang Z, Qi X, Song G
Arteriosclerosis, thrombosis, and vascular biology 2023 Jun;43(6):995-1014

Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-κB signaling pathway.

He Y, Wang Y, Jia X, Li Y, Yang Y, Pan L, Zhao R, Han Y, Wang F, Guan X, Hou T
International immunopharmacology 2023 Jun;119:110192

Induced neural stem cells suppressed neuroinflammation by inhibiting the microglial pyroptotic pathway in intracerebral hemorrhage rats.

Liu J, Cao C, Jin Y, Wang Y, Ma X, Li J, Guo S, Yang J, Niu J, Liang X
iScience 2023 Jul 21;26(7):107022

The Correlation between Increased Expressions of NLRP3 Inflammasome Components in Peripheral Blood Mono-Nuclear Cells and Plaque Vulnerability in Human Carotid Atherosclerosis.

Xie W, Shi X, Lee H, Wang L, Niu N, Qu P
Iranian journal of public health 2023 Jan;52(1):147-158

TMBIM4 Deficiency Facilitates NLRP3 Inflammasome Activation-Induced Pyroptosis of Trophoblasts: A Potential Pathogenesis of Preeclampsia.

Chen Y, Xiao L, Sun G, Li M, Yang H, Ming Z, Zhao K, Shang X, Zhang H, Liu C
Biology 2023 Jan 29;12(2)

Alpha-kinase1 promotes tubular injury and interstitial inflammation in diabetic nephropathy by canonical pyroptosis pathway.

Cui X, Li Y, Yuan S, Huang Y, Chen X, Han Y, Liu Z, Li Z, Xiao Y, Wang Y, Sun L, Liu H, Zhu X
Biological research 2023 Feb 2;56(1):5

Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome.

Shi X, Sun Q, Hou Y, Zeng H, Cao Y, Dong M, Ding J, Shao F
Nature 2023 Dec;624(7991):442-450

Inhibition of macrophage pyroptosis ameliorates silica-induced pulmonary fibrosis.

Jiang F, Jiang Q, Hou L, Zhao J, Zhu Z, Jia Q, Xue W, Wang H, Wang Y, Tian L
Ecotoxicology and environmental safety 2023 Dec;268:115693

Inhibition of Dectin-1 Alleviates Neuroinflammatory Injury by Attenuating NLRP3 Inflammasome-Mediated Pyroptosis After Intracerebral Hemorrhage in Mice: Preliminary Study Results.

Ding Z, Zhong Z, Wang J, Zhang R, Shao J, Li Y, Wu G, Tu H, Yuan W, Sun H, Wang Q
Journal of inflammation research 2022;15:5917-5933

Tongluo Yishen Decoction Ameliorates Renal Fibrosis via NLRP3-Mediated Pyroptosis In Vivo and In Vitro.

Jia Q, Zhang X, Hao G, Zhao Y, Lowe S, Han L, Qin J
Frontiers in pharmacology 2022;13:936853

Methamphetamine Disturbs Gut Homeostasis and Reshapes Serum Metabolome, Inducing Neurotoxicity and Abnormal Behaviors in Mice.

Zhang KK, Chen LJ, Li JH, Liu JL, Wang LB, Xu LL, Yang JZ, Li XW, Xie XL, Wang Q
Frontiers in microbiology 2022;13:755189

β-Hydroxybutyrate against Cisplatin-Induced acute kidney injury via inhibiting NLRP3 inflammasome and oxidative stress.

Luo S, Yang M, Han Y, Zhao H, Jiang N, Li L, Chen W, Li C, Yang J, Liu Y, Liu C, Zhao C, Sun L
International immunopharmacology 2022 Oct;111:109101

Cathepsin B/HSP70 complex induced by Ilexsaponin I suppresses NLRP3 inflammasome activation in myocardial ischemia/reperfusion injury.

Wu J, Chen S, Wu P, Wang Y, Qi X, Zhang R, Liu Z, Wang D, Cheng Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Oct;105:154358

Aerobic exercise training alleviates renal injury in db/db mice through inhibiting Nox4-mediated NLRP3 inflammasome activation.

Zhou Z, Ying C, Zhou X, Shi Y, Xu J, Zhu Y, Wang M, Li Y, Li X, Xiang J
Experimental gerontology 2022 Oct 15;168:111934

GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome.

Qin Z, Song J, Lin A, Yang W, Zhang W, Zhong F, Huang L, Lü Y, Yu W
Journal of neuroinflammation 2022 May 27;19(1):121

Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4(+) T cells in chronic liver injury.

Zhang Y, Yang X, Bi T, Wu X, Wang L, Ren Y, Ou Y, Xie C, Li K, Ran H, Wang J, Zhao F, Shui P, Qing J
International immunopharmacology 2022 Jun;107:108639

Role of salivary glycopatterns for oral microbiota associated with gastric cancer.

Shu J, Yu H, Ren X, Wang Y, Zhang K, Tang Z, Dang L, Chen W, Li B, Xie H, Li Z
International journal of biological macromolecules 2022 Jun 1;209(Pt A):1368-1378

METTL3 overexpression aggravates LPS-induced cellular inflammation in mouse intestinal epithelial cells and DSS-induced IBD in mice.

Yang L, Wu G, Wu Q, Peng L, Yuan L
Cell death discovery 2022 Feb 14;8(1):62

MCC950 ameliorates ventricular arrhythmia vulnerability induced by heart failure.

Jiang X, Yang F, Ou D, Huang L, Li H, Lang M
Bioengineered 2022 Apr;13(4):8593-8604

Exploring Molecular Mechanisms Involved in the Development of the Depression-Like Phenotype in Interleukin-18-Deficient Mice.

Yamanishi K, Miyauchi M, Mukai K, Hashimoto T, Uwa N, Seino H, Li W, Gamachi N, Hata M, Kuwahara-Otani S, Maeda S, Watanabe Y, Yamanishi H, Yagi H, Okamura H, Matsunaga H
BioMed research international 2021;2021:9975865

Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer.

Yan H, Luo B, Wu X, Guan F, Yu X, Zhao L, Ke X, Wu J, Yuan J
International journal of biological sciences 2021;17(10):2606-2621

Resatorvid Relieves Breast Cancer Complicated with Depression by Inactivating Hippocampal Microglia Through TLR4/NF-κB/NLRP3 Signaling Pathway.

Luo W, Han Y, Meng P, Yang Q, Zhao H, Ling J, Wang Y
Cancer management and research 2020;12:13003-13014

Endogenous Interleukin 18 Suppresses Liver Regeneration After Hepatectomy in Mice.

Ma T, Zhang Y, Lao M, Chen W, Hu Q, Zhi X, Chen Z, Bai X, Dang X, Liang T
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society 2020 Mar;26(3):408-418

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 60070-1-Ig( Pre-IL18 Antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 60070-1-Ig( Pre-IL18 Antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using IL18 antibody 60070-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using IL18 antibody 60070-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Sample type: cell line