60070-1-Ig

antibody from Proteintech Group

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Provider product page for 60070-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [56]
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Validations
Western blot [2]
Immunocytochemistry [2]

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Product number: 60070-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#60070-1-Ig, RRID:AB_2280158
Product name: IL-18 antibody
Antibody type: Monoclonal
Description: KD/KO validated IL-18 antibody (Cat. #60070-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, hamster and has been validated for the following applications: IHC, WB,ELISA.
Reactivity: Human, Hamster
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 5C6F8
Vial size: 20ul, 150ul

IL18 protein structure - 60070-1-Ig shown in red.

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Morani F, Dell'Anno I, Daga A, Doccini S, Bisceglia L, Gemignani F, Landi S
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Targeting the RAGE-RIPK1 binding site attenuates diabetes-associated cognitive deficits.

Gao L, Wu S, Hu B, Zhang Q, Wu Y, Li H, Qian Y, Huang C, Wen X, Li H, Cheng A, Song Y, Ying C, Zhou X
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Mast cells boost anti-tumor potency of MAIT cells via inflammasome-dependent secretion of IL-18.

Fan F, Wang J, Liu K, Zhang S, Gao J, Li X, Ma J, Zhao Y, Li T, Su H, Yang X, Han H, Huang Q, Zhang Y, Pan Y, Ye T, Hu H, Sun Y, Li F, Cao Z, Zhang Y, Zhang X, Chen H
Nature communications 2025 Jul 2;16(1):6074

BMP6, a potential biomarker of inflammatory fibrosis and promising protective factor for dilated cardiomyopathy.

Jiang F, Tang J, Wei X, Pan H, Fan X, Zhang P, Guo S
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Yang J, Wang M, Shi L, Fang X, Gao C, Ma L, Wang Y, Ying S, Yang Y
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Zhou M, Wang B, Cai S, Wei T, Zhang Y, Fang L, Nie S, Wang B, Xiao B, Xiao J, Wu Y
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Shi Y, Zhao Y, Sun SJ, Lan XT, Wu WB, Zhang Z, Chen YX, Yan YY, Xu YP, Li DJ, Fu H, Shen FM
Biochemical pharmacology 2025 Apr;234:116800

Attenuation of NLRP3 Inflammasome by Cigarette Smoke is Correlated with Decreased Defense Response of Oral Epithelial Cells to Candida albicans.

Huang F, Xie R, Li R, Liu L, Zhao M, Wang Q, Liu W, Ye P, Wang W, Wang X
Current molecular medicine 2024;24(6):790-800

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Yao M, Fang C, Wang Z, Guo T, Wu D, Ma J, Wu J, Mo J
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Ji C, Hao X, Li Z, Liu J, Yan H, Ma K, Li L, Zhang L
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Xu L, Yang X, Liu XT, Li XY, Zhu HZ, Xie YH, Wang SW, Li Y, Zhao Y
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Zhang X, Huo Z, Jia X, Xiong Y, Li B, Zhang L, Li X, Li X, Fang Y, Dong X, Chen G
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The European journal of neuroscience 2024 Jan;59(2):208-219

Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and reperfusion--From the perspective of glial-vascular unit.

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Long non-coding RNA NEAT1 exacerbates NLRP3-mediated pyroptosis in allergic rhinitis through regulating the PTBP1/FOXP1 cascade.

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Transcutaneous electrical acupoint stimulation alleviates cerebral ischemic injury through the TLR4/MyD88/NF-κ B pathway.

Wu L, Tan Z, Su L, Dong F, Xu G, Zhang F
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PACS-2 deficiency aggravates tubular injury in diabetic kidney disease by inhibiting ER-phagy.

Yang J, Li L, Li C, Chen W, Liu Y, Luo S, Zhao C, Han Y, Yang M, Zhao H, Jiang N, Xi Y, Tang C, Cai J, Xiao L, Liu H, Sun L
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Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-κB signaling pathway.

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International immunopharmacology 2023 Jun;119:110192

Induced neural stem cells suppressed neuroinflammation by inhibiting the microglial pyroptotic pathway in intracerebral hemorrhage rats.

Liu J, Cao C, Jin Y, Wang Y, Ma X, Li J, Guo S, Yang J, Niu J, Liang X
iScience 2023 Jul 21;26(7):107022

The Correlation between Increased Expressions of NLRP3 Inflammasome Components in Peripheral Blood Mono-Nuclear Cells and Plaque Vulnerability in Human Carotid Atherosclerosis.

Xie W, Shi X, Lee H, Wang L, Niu N, Qu P
Iranian journal of public health 2023 Jan;52(1):147-158

TMBIM4 Deficiency Facilitates NLRP3 Inflammasome Activation-Induced Pyroptosis of Trophoblasts: A Potential Pathogenesis of Preeclampsia.

Chen Y, Xiao L, Sun G, Li M, Yang H, Ming Z, Zhao K, Shang X, Zhang H, Liu C
Biology 2023 Jan 29;12(2)

Alpha-kinase1 promotes tubular injury and interstitial inflammation in diabetic nephropathy by canonical pyroptosis pathway.

Cui X, Li Y, Yuan S, Huang Y, Chen X, Han Y, Liu Z, Li Z, Xiao Y, Wang Y, Sun L, Liu H, Zhu X
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Shi X, Sun Q, Hou Y, Zeng H, Cao Y, Dong M, Ding J, Shao F
Nature 2023 Dec;624(7991):442-450

Inhibition of macrophage pyroptosis ameliorates silica-induced pulmonary fibrosis.

Jiang F, Jiang Q, Hou L, Zhao J, Zhu Z, Jia Q, Xue W, Wang H, Wang Y, Tian L
Ecotoxicology and environmental safety 2023 Dec;268:115693

Inhibition of Dectin-1 Alleviates Neuroinflammatory Injury by Attenuating NLRP3 Inflammasome-Mediated Pyroptosis After Intracerebral Hemorrhage in Mice: Preliminary Study Results.

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Tongluo Yishen Decoction Ameliorates Renal Fibrosis via NLRP3-Mediated Pyroptosis In Vivo and In Vitro.

Jia Q, Zhang X, Hao G, Zhao Y, Lowe S, Han L, Qin J
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Methamphetamine Disturbs Gut Homeostasis and Reshapes Serum Metabolome, Inducing Neurotoxicity and Abnormal Behaviors in Mice.

Zhang KK, Chen LJ, Li JH, Liu JL, Wang LB, Xu LL, Yang JZ, Li XW, Xie XL, Wang Q
Frontiers in microbiology 2022;13:755189

β-Hydroxybutyrate against Cisplatin-Induced acute kidney injury via inhibiting NLRP3 inflammasome and oxidative stress.

Luo S, Yang M, Han Y, Zhao H, Jiang N, Li L, Chen W, Li C, Yang J, Liu Y, Liu C, Zhao C, Sun L
International immunopharmacology 2022 Oct;111:109101

Cathepsin B/HSP70 complex induced by Ilexsaponin I suppresses NLRP3 inflammasome activation in myocardial ischemia/reperfusion injury.

Wu J, Chen S, Wu P, Wang Y, Qi X, Zhang R, Liu Z, Wang D, Cheng Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Oct;105:154358

Aerobic exercise training alleviates renal injury in db/db mice through inhibiting Nox4-mediated NLRP3 inflammasome activation.

Zhou Z, Ying C, Zhou X, Shi Y, Xu J, Zhu Y, Wang M, Li Y, Li X, Xiang J
Experimental gerontology 2022 Oct 15;168:111934

GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome.

Qin Z, Song J, Lin A, Yang W, Zhang W, Zhong F, Huang L, Lü Y, Yu W
Journal of neuroinflammation 2022 May 27;19(1):121

Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4(+) T cells in chronic liver injury.

Zhang Y, Yang X, Bi T, Wu X, Wang L, Ren Y, Ou Y, Xie C, Li K, Ran H, Wang J, Zhao F, Shui P, Qing J
International immunopharmacology 2022 Jun;107:108639

Role of salivary glycopatterns for oral microbiota associated with gastric cancer.

Shu J, Yu H, Ren X, Wang Y, Zhang K, Tang Z, Dang L, Chen W, Li B, Xie H, Li Z
International journal of biological macromolecules 2022 Jun 1;209(Pt A):1368-1378

METTL3 overexpression aggravates LPS-induced cellular inflammation in mouse intestinal epithelial cells and DSS-induced IBD in mice.

Yang L, Wu G, Wu Q, Peng L, Yuan L
Cell death discovery 2022 Feb 14;8(1):62

MCC950 ameliorates ventricular arrhythmia vulnerability induced by heart failure.

Jiang X, Yang F, Ou D, Huang L, Li H, Lang M
Bioengineered 2022 Apr;13(4):8593-8604

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Yamanishi K, Miyauchi M, Mukai K, Hashimoto T, Uwa N, Seino H, Li W, Gamachi N, Hata M, Kuwahara-Otani S, Maeda S, Watanabe Y, Yamanishi H, Yagi H, Okamura H, Matsunaga H
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Yan H, Luo B, Wu X, Guan F, Yu X, Zhao L, Ke X, Wu J, Yuan J
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Luo W, Han Y, Meng P, Yang Q, Zhao H, Ling J, Wang Y
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Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society 2020 Mar;26(3):408-418

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 60070-1-Ig( Pre-IL18 Antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 60070-1-Ig( Pre-IL18 Antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using IL18 antibody 60070-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using IL18 antibody 60070-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Sample type: cell line