Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF521 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-18/IL-1F4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat IL-18/IL-1F4 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse IL-18 is observed and approximately 10% cross-reactivity with recombinant human IL-18 is observed.
- Reactivity
- Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain.
Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.
Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
Role of IL-18 in acute lung inflammation.
Role of IL-18 in acute lung inflammation.
Kuwahara-Otani S, Maeda S, Kobayashi K, Minato Y, Tanaka K, Yamanishi K, Hata M, Li W, Hayakawa T, Noguchi K, Okamura H, Yagi H
Neuroscience letters 2017 May 22;650:33-37
Neuroscience letters 2017 May 22;650:33-37
Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.
Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Nov 26;28(48):12775-87
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Nov 26;28(48):12775-87
Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK 3rd, Woods JM, Koch AE
Arthritis and rheumatism 2006 Aug;54(8):2402-14
Arthritis and rheumatism 2006 Aug;54(8):2402-14
Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Bührer C
Annals of neurology 2005 Jan;57(1):50-9
Annals of neurology 2005 Jan;57(1):50-9
Role of IL-18 in acute lung inflammation.
Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2001 Dec 15;167(12):7060-8
Journal of immunology (Baltimore, Md. : 1950) 2001 Dec 15;167(12):7060-8
Role of IL-18 in acute lung inflammation.
Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2001 Dec 15;167(12):7060-8
Journal of immunology (Baltimore, Md. : 1950) 2001 Dec 15;167(12):7060-8
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutralization by Rat IL-18/IL-1F4 Antibody. In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Rat IL-18/IL-1F4 (Catalog # 521-RL) stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Rat IL-18/IL-1F4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF521). The ND50 is typically 1-3 µg/mL.