F52493

antibody from NSJ Bioreagents

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F52493

Provider

NSJ Bioreagents

Product name

IL-18 Antibody

Antibody type

Polyclonal

Antigen

This IL-18 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 155-188 amino acids from the C-terminal region of human IL18.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the IL-18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from HeLa cell line using IL-18 antibody. Predicted molecular weight: 17~24 kDa

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of U251 cells stained with IL-18 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human small intestine section using IL-18 antibody

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse small intestine section using IL-18 antibody

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rat small intestine section using IL-18 antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using IL-18 antibody (green) compared to an isotype control of rabbit IgG (blue); Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.